Department of Environmental Science and Engineering, Center for Environmental Studies, Kyung Hee University, Seochon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
Bioresour Technol. 2012 Jan;103(1):136-41. doi: 10.1016/j.biortech.2011.09.093. Epub 2011 Oct 8.
A batch test and continuous operation were performed to identify the effect of lactate on hydrogen production at pH 4.5. When the initial lactic acid concentration was increased from 0 to 8 g/L in the batch test, the hydrogen yield also increased from 1.41 to 1.72 mol-H2/mol-glucose. The system exhibited a long lag time and an insignificant hydrogen yield with 16 g-lactic acid/L. A continuous stirred tank reactor (CSTR) was operated at different organic loading rates (OLRs: 10, 15, 20 and 40 g/L/day) and hydraulic retention times (HRTs: 6, 12 and 24 h). At an OLR of 20 g-glucose/L/day and 12 h of HRT, the hydrogen yield was 1.2 mol-H2/mol-glucose. The yield decreased with a 24 h HRT. Even though lactate was one of the major constituents of volatile fatty acids (VFAs), hydrogen production was feasible throughout the operation. Clostridium sp. was the dominant hydrogen-producing bacteria in the system.
进行了分批试验和连续操作，以确定乳酸在 pH4.5 下对氢气生产的影响。当分批试验中初始乳酸浓度从 0 增加到 8 g/L 时，氢气产量也从 1.41 增加到 1.72 mol-H2/mol-葡萄糖。当乳酸浓度为 16 g/L 时，系统表现出较长的迟滞时间和微不足道的氢气产量。连续搅拌槽式反应器 (CSTR) 在不同的有机负荷率 (OLR：10、15、20 和 40 g/L/天) 和水力停留时间 (HRT：6、12 和 24 h) 下运行。在 OLR 为 20 g-葡萄糖/L/天时和 12 h 的 HRT 下，氢气产量为 1.2 mol-H2/mol-葡萄糖。随着 HRT 增加到 24 h，产氢量下降。尽管乳酸是挥发性脂肪酸 (VFAs) 的主要成分之一，但在整个运行过程中仍可进行氢气生产。产氢菌属是系统中的主要产氢细菌。
