L'vov K M, Bekmurzaev B M
Biofizika. 1990 May-Jun;35(3):421-4.
Accumulation of radicals UV-induced in protein solutions at 77 K was investigated. It has been found that in each molecule of small proteins true solutions only one ionization proceeds and two radicals are formed. The limiting number of radicals per one protein molecule decreases with the increase of their aggregation level. A method is suggested for observing aggregation and desegregation processes in proteins, as well as a method for determining the absolute concentration of radicals.
研究了77K下紫外线诱导蛋白质溶液中自由基的积累。已发现，在小蛋白质真溶液的每个分子中，仅发生一次电离并形成两个自由基。每个蛋白质分子的自由基极限数量随着其聚集水平的增加而减少。提出了一种观察蛋白质中聚集和解聚过程的方法，以及一种测定自由基绝对浓度的方法。
