Krivandin A V, Feĭgin L A
Biofizika. 1990 May-Jun;35(3):461-3.
X-ray small-angle scattering study of bovine lens tissue was carried out. It was shown that X-ray patterns of lens cortical and nuclear native tissues did not contain the small-angle maxima. The maximum in the range of 15-20 nm Bragg distance appeared as a result of the lens tissue partial dehydration. Earlier such maximum was considered by some authors as the evidence of crystallin proteins short-range order in the native lens. Now it is confirmed to be a preparative artefact of dehydration. It was shown also that similar maximum in 15-20 range existed in the case of concentrated crystallin solutions. This indicates that supramolecular organization of crystallins in the native lens is not similar to that in the concentrated solution.
对牛晶状体组织进行了X射线小角散射研究。结果表明,晶状体皮质和核天然组织的X射线图谱中不包含小角最大值。在15 - 20纳米布拉格距离范围内出现的最大值是晶状体组织部分脱水的结果。此前,一些作者认为这种最大值是天然晶状体中晶状体蛋白短程有序的证据。现在已证实这是脱水的制备假象。还表明,在浓缩晶状体蛋白溶液的情况下,也存在15 - 20范围内的类似最大值。这表明天然晶状体中晶状体蛋白的超分子组织与浓缩溶液中的不同。