Krivandin A V, L'vov Iu M, Ostrovskiĭ M A, Fedorovich I B, Feĭgin L A
Biofizika. 1984 Sep-Oct;29(5):873-7.
X-ray diffraction method has been applied for investigating ocular lens native tissue of the frog. X-ray diffraction patterns of intact lenses, their nuclei and cortices are similar and contain a set of concentric diffuse diffraction maxima. The most intensive of these maxima corresponding to the Bragg-spacings of 14.6, 9.1 and 4.6 A are presumably associated with intramolecular structure of lens proteins--crystallins. Intensive small-angle X-ray scattering and diffraction patterns isotropy indicates unavailability of crystallin molecule ordering or orientation in the lens. The shift of 14.6 A maximum up to 12.8 A being the result of nuclei drying shows the necessity of aqueous surrounding for these protein native structure maintenance.
X射线衍射法已被用于研究青蛙的眼晶状体天然组织。完整晶状体、其细胞核和皮质的X射线衍射图谱相似，且包含一组同心的漫射衍射最大值。这些最大值中最强的对应于14.6、9.1和4.6埃的布拉格间距，推测与晶状体蛋白——晶状体球蛋白的分子内结构有关。强烈的小角X射线散射和衍射图谱各向同性表明晶状体中晶状体球蛋白分子不存在有序排列或取向。细胞核干燥导致14.6埃最大值向12.8埃的移动表明，为维持这些蛋白质的天然结构需要水的环境。
