Stitnimankarn T, Thakerngpol K, Damrongsak C, Chinapak O, Sindhvananda K
Southeast Asian J Trop Med Public Health. 1978 Dec;9(4):558-67.
An electron microscopic study was carried out on eleven surgical liver biopsy specimens obtained from patients with cholangiocarcinoma associated with opisthorchiasis. The tumor cells of histologically well differentiated cholangiocarcinoma had few cytoplasmic organelles. They contained relatively large nuclei, abundant free ribosomes and numerous groups of fine fibrils. Each cell was surrounded by a basement membrane. Numerous long microvilli were seen projecting into the glandular lumen. The moderately differentiated cholangiocarcinomatous cells exhibited increased organelle content, marked variation in the shape of the nuclei with deep cytoplasmic invagination into the nuclear membrane; there were small intranuclear pseudoinclusions, and shorter microvilli. The tumor cells showed intracellular microvillus-lined spaces, abundant free ribosomes, many fine fibrils and their surrounding basement membranes were incomplete. The ultrastructure of the poorly differentiated cholangiocarcinoma was similar to that of the moderately differentiated tumor, except for fewer microvilli, abundant cytoplasmic organelles, and ill-defined or absent basement membrane.
对11例从华支睾吸虫病相关性胆管癌患者获取的手术肝脏活检标本进行了电子显微镜研究。组织学上高分化胆管癌的肿瘤细胞胞质细胞器较少。它们含有相对较大的细胞核、丰富的游离核糖体和大量的细纤维束。每个细胞都被基底膜包围。可见许多长微绒毛伸入腺腔。中分化胆管癌细胞的细胞器含量增加，细胞核形状有明显变化，细胞质深深内陷至核膜；有小的核内假包涵体，微绒毛较短。肿瘤细胞显示有细胞内微绒毛衬里的间隙，游离核糖体丰富，有许多细纤维，其周围基底膜不完整。低分化胆管癌的超微结构与中分化肿瘤相似，只是微绒毛较少，胞质细胞器丰富，基底膜不清晰或缺失。
