Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong Province, China.
Acta Trop. 2012 Feb;121(2):118-24. doi: 10.1016/j.actatropica.2011.10.013. Epub 2011 Nov 2.
Each of BALB/c mice was infected with 50 Angiostrongylus cantonensis larvae. One group of mice received an intraperitoneal injection of 50 μg 12D5 monoclonal antibody (mAb) against a 98 kDa antigen of adult worms at 10 days post-infection (dpi), with a booster injection of 25 μg at 12 dpi. Five mice from each group were sacrificed at 14 dpi for pathological examination and RNA extraction. The infiltration of eosinophils and severity of eosinophilic meningitis were reduced in 12D5 mAb-treated mice compared with the infected mice without 12D5 treatment. The levels of eotaxin mRNA expression in spleen significantly increased and the expression of the Th2-type cytokine IL-5 significantly decreased. However, the expression of IL-4 was not changed. 12D5 mAb can observably enhance the survival rate of infected mice and reduce symptoms of angiostrongyliasis. A. cantonensis infection is a major cause of eosinophilic meningoencephalitis. The results of this study could be helpful for the development of treatment of human angiostrongylosis.
每只 BALB/c 小鼠感染 50 条广州管圆线虫幼虫。一组小鼠在感染后 10 天（dpi）接受 50 μg 针对成虫 98 kDa 抗原的 12D5 单克隆抗体（mAb）腹腔内注射，并在 12 dpi 时进行 25 μg 的加强注射。每组中有 5 只小鼠在 14 dpi 时用于病理检查和 RNA 提取。与未用 12D5 处理的感染小鼠相比，12D5 mAb 处理的小鼠中嗜酸性粒细胞浸润和嗜酸性粒细胞性脑膜炎的严重程度降低。脾组织中 eotaxin mRNA 表达水平显著增加，Th2 型细胞因子 IL-5 表达显著降低，但 IL-4 表达没有变化。12D5 mAb 可明显提高感染小鼠的存活率并减轻旋毛虫病的症状。广州管圆线虫感染是嗜酸性粒细胞性脑膜脑炎的主要原因。本研究结果可能有助于开发人类旋毛虫病的治疗方法。
