Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Kawasaki, Kanagawa 216-8511, Japan.
Reprod Toxicol. 2012 Jan;33(1):27-34. doi: 10.1016/j.reprotox.2011.10.010. Epub 2011 Oct 29.
A potent neurotoxin 3,3'-iminodipropionitrile (IDPN), which is an occupational exposure hazard in industry, induces persistent behavioral abnormalities in experimental animals; however, its reproductive toxicity has not been determined. Therefore, we assessed the toxicity of IDPN in the reproductive system of female rats. A single intraperitoneal injection of IDPN (1000 mg/kg body weight) into female Wistar-Imamichi rats caused acute estrous cycle arrest at diestrus for up to 15 days. The arrest was accompanied by follicular atresia, and following arrest, the estrous cycle and ovarian morphology recovered. Ovarian mRNA levels of growth differentiation factor 9 and Fas ligand, a cell death marker, transiently increased following IDPN injection, but eventually they returned to basal levels. IDPN added to in vitro cultures of ovarian follicles also induced the expression of these genes, indicating that IDPN directly promoted ovarian cell death.
一种强效神经毒素 3,3'-亚氨基二丙腈（IDPN），作为工业职业暴露危害因素，可在实验动物中引起持续的行为异常；然而，其生殖毒性尚未确定。因此，我们评估了 IDPN 对雌性大鼠生殖系统的毒性。单次腹腔注射 IDPN（1000mg/kg 体重）可导致 Wistar-Imamichi 雌性大鼠急性发情周期停止，最长可达 15 天。这种抑制伴随着卵泡闭锁，抑制后发情周期和卵巢形态恢复。IDPN 注射后，卵巢生长分化因子 9 和 Fas 配体（一种细胞死亡标志物）的卵巢 mRNA 水平短暂增加，但最终恢复到基础水平。IDPN 添加到卵巢卵泡的体外培养物中也诱导这些基因的表达，表明 IDPN 直接促进卵巢细胞死亡。
