咖啡因在未处理聚氨酯泡沫上的吸附特性：用于其在人体血浆中的测定
Sorption characteristics of caffeine onto untreated polyurethane foam: application to its determination in human plasma.
作者信息
Abdel Azeem Samy M, Ali Samah, El-Shahat Mohamed F
机构信息
Chemistry Department, Faculty of Science, Fayoum University, Fayoum, Egypt.
出版信息
Anal Sci. 2011;27(11):1133-7. doi: 10.2116/analsci.27.1133.
In the present paper, the sorption properties of caffeine (CAF) onto polyether type polyurethane foam (PUF) as solid phase sorbent were investigated with UV determination at 274.3 nm. Batch and column methods were used to optimize chemical, flow, kinetic and isothermal conditions for preconcentration of CAF. Results indicated quantitative sorption of CAF at pH 8 and 30 min shaking time. The maximum sorption capacity was found to be 4.1 mg g(-1). Column preconcentration was recommended at a flow rate of 1.5 mL min(-1) and desorption with 4 mL from 0.15 mol L(-1) hydrochloric acid. The procedure provided a linear analytical range of 0.05-30 mg L(-1). The detection and quantification limits are 0.016 and 0.047 mg L(-1), respectively. The procedure was applied to determination of CAF in spiked human plasma. The obtained recoveries were 98-101% and RSD values were from 0.05 to 9.5%.
在本论文中，以紫外检测法在274.3 nm波长下研究了咖啡因（CAF）在作为固相吸附剂的聚醚型聚氨酯泡沫（PUF）上的吸附特性。采用批次法和柱法对CAF预富集的化学、流速、动力学和等温条件进行优化。结果表明，在pH值为8且振荡时间为30分钟时，CAF可实现定量吸附。最大吸附容量为4.1 mg g(-1)。建议柱预富集流速为1.5 mL min(-1)，并用4 mL 0.15 mol L(-1)盐酸进行解吸。该方法的线性分析范围为0.05 - 30 mg L(-1)。检测限和定量限分别为0.016和0.047 mg L(-1)。该方法应用于加标人血浆中CAF的测定。获得的回收率为98 - 101%，相对标准偏差值为0.05至9.5%。
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