锌试剂固定化聚氨酯泡沫预富集后水中和生物样品中锌的在线测定
On-line Determination of Zinc in Water and Biological Samples after Its Preconcentration onto Zincon Anchored Polyurethane Foam.
作者信息
Azeem Sami M Abdel, Hanafi Hassan A, El-Shahat M F
机构信息
Chemistry Department, Faculty of Science, Fayoum University, 2) Collage of Science and Humanities-Al-Quwaiyah, Shaqra University-KSA.
出版信息
Anal Sci. 2015;31(5):391-7. doi: 10.2116/analsci.31.391.
A fast and sensitive on-line procedure for the determination of zinc in water and biological samples was developed. Zinc was preconcentrated in a mini-column packed with polyurethane foam (PUF) chemically modified with zincon via -N=N- bonding. The optimal conditions for preconcentration were pH 8.5 and sample flow rate of 4.0 mL min(-1). Quantitative desorption of Zn(II) was obtained by 0.1 mol L(-1) hydrochloric acid and subsequent spectrophotmetric determination using 4-(2-pyridylazo)-resorcinol at 498 nm. The obtained detection limit was found to be 3.0 ng mL(-1), precision (RSD) was 4.8 and 6.7% at 20 and 110 ng mL(-1), respectively, for 60 s preconcentration time and enrichment factor was 31. The linearity range was from 10 to 120 ng mL(-1) and maximum sample throughput was 20 h(-1). Finally, the method was successfully applied to the determination of zinc in tap water, Nile River water and human urine samples with RSD in the range of 1.1 - 8.3%.
开发了一种快速灵敏的在线方法用于测定水和生物样品中的锌。锌通过 -N=N- 键合在填充有经锌试剂化学修饰的聚氨酯泡沫（PUF）的微型柱中进行预富集。预富集的最佳条件为pH 8.5和样品流速4.0 mL min⁻¹。用0.1 mol L⁻¹盐酸对Zn(II)进行定量解吸，随后使用4-(2-吡啶偶氮)-间苯二酚在498 nm处进行分光光度测定。获得的检测限为3.0 ng mL⁻¹，对于60 s的预富集时间，在20和110 ng mL⁻¹时精密度（相对标准偏差，RSD）分别为4.8%和6.7%，富集因子为31。线性范围为10至120 ng mL⁻¹，最大样品通量为20 h⁻¹。最后，该方法成功应用于自来水、尼罗河水和人尿样品中锌的测定，RSD在1.1 - 8.3%范围内。
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