Suzuki H, Yamamoto T, Yamamoto H, Konno H, Iwasaki Y, Ohara Y, Terunuma H
Department of Neurological Sciences, Tohoku University School of Medicine, Japan.
Brain Res. 1990 Jun 18;520(1-2):324-9. doi: 10.1016/0006-8993(90)91723-t.
Because laminin possesses potent neurotrophic activity, laminin-like immunoreactivity was investigated in formalin-fixed human brains, using affinity-purified anti-human placental laminin rabbit serum. Distinct laminin-like immunoreactivity was observed in neuronal soma of certain nuclear groups, and a 180-kDa band was noted in an immunoblot study of the supernatant of brain homogenate. These observations imply that many but not all central neurons have laminin-like immunoreactive molecules. Their functional role, however, remains to be clarified.
由于层粘连蛋白具有强大的神经营养活性，因此使用亲和纯化的抗人胎盘层粘连蛋白兔血清，在福尔马林固定的人脑中研究了层粘连蛋白样免疫反应性。在某些核团的神经元胞体中观察到明显的层粘连蛋白样免疫反应性，并且在脑匀浆上清液的免疫印迹研究中发现了一条180 kDa的条带。这些观察结果表明，许多但并非所有中枢神经元都具有层粘连蛋白样免疫反应性分子。然而，它们的功能作用仍有待阐明。
