Department of Pathology, Korea University College of Medicine, and Korea University Guro Hospital, Seongbuk-ku, Seoul 136-701, Republic of Korea.
Oncol Rep. 2012 Mar;27(3):608-20. doi: 10.3892/or.2011.1545. Epub 2011 Nov 10.
The study of tumor biomarkers was gradually facilitated by the adoption of proteomic strategies due to less invasiveness and higher sensitivity. Colorectal cancer is one of the most commonly occurring cancers worldwide and its incidence has markedly increased in Korea. While the adoption of proteomic strategies facilitated the study of tumor biomarkers, to date, no common agreement has been derived from proteomic investigations regarding tumor markers of colorectal cancer. This study was designed to find molecules differentially expressed in colorectal cancer compared to non-tumor mucosa. Four colorectal adenocarcinoma and corresponding non-tumor tissue samples were analyzed to find previously unknown proteins via two-dimensional electrophoresis and MALDI-TOF/MS spectrometry. Western blot assays and tissue microarray (TMA) immunohistochemistry were performed to validate the identified proteins. Among the twelve up-regulated and one down-regulated proteins identified, moesin, cytokeratin (KRT) 17 and carbonic anhydrase I were validated by western blot analysis and/or immunohistochemistry. On immunohistochemistry, both moesin and KRT17 demonstrated a tendency of increased expression as pT stage advanced. Both moesin and KRT17 were not expressed in normal colorectal epithelium. These two proteins may play a role in cancer invasion and/or metastasis in colorectal carcinoma, and could be candidate biomarkers for the diagnosis and prognosis of colorectal cancer.
由于具有侵袭性低和灵敏度高的特点，蛋白质组学策略的采用逐渐促进了肿瘤标志物的研究。结直肠癌是全世界最常见的癌症之一，在韩国其发病率显著增加。尽管蛋白质组学策略的采用促进了肿瘤标志物的研究，但迄今为止，蛋白质组学研究尚未得出关于结直肠癌肿瘤标志物的共识。本研究旨在寻找与非肿瘤黏膜相比在结直肠癌中差异表达的分子。通过二维电泳和 MALDI-TOF/MS 质谱分析了 4 例结直肠腺癌和相应的非肿瘤组织样本，以发现以前未知的蛋白质。通过 Western blot 检测和组织微阵列（TMA）免疫组织化学验证鉴定的蛋白。在鉴定的 12 个上调和 1 个下调蛋白中，通过 Western blot 分析和/或免疫组织化学验证了膜突蛋白、细胞角蛋白（KRT）17 和碳酸酐酶 I。在免疫组织化学中，moesin 和 KRT17 均随着 pT 期的进展表现出表达增加的趋势。moesin 和 KRT17 在正常结直肠上皮中均不表达。这两种蛋白可能在结直肠癌的侵袭和/或转移中发挥作用，可作为结直肠癌诊断和预后的候选生物标志物。
