Ginzburg R, Seltzer Z
Physiology Branch, Faculty of Dental Medicine, Hebrew University of Jerusalem, Israel.
Brain Res. 1990 Jul 16;523(1):147-50. doi: 10.1016/0006-8993(90)91649-2.
Several weeks after transection of the sciatic and saphenous nerves, rats respond by self-injury of the denervated limb ('autotomy'). This behavior serves as a model of neuropathic pain. In this study we allografted fragments of rat adrenal medulla into the subarachnoid space of other rats, at lumbar spinal cord level, in an attempt to suppress autotomy behavior. The results show that autotomy was reduced by an average of 63% throughout the 8 week observation period. Catecholamine (CA) histofluorescent staining performed up to 120 days postoperatively (P0) revealed viable transplants in 75% of the rats. Transplant viability correlated with suppression of autotomy. This suggests that medullary chromaffin cells function as a local, long-lasting source of anti-nociceptive agents at the spinal segments which process input from the injured nerves.
坐骨神经和隐神经横断数周后，大鼠会出现去神经肢体的自我伤害行为（“自残”）。这种行为可作为神经性疼痛的模型。在本研究中，我们将大鼠肾上腺髓质碎片移植到其他大鼠腰椎脊髓水平的蛛网膜下腔，试图抑制自残行为。结果显示，在整个8周的观察期内，自残行为平均减少了63%。术后长达120天（P0）进行的儿茶酚胺（CA）组织荧光染色显示，75%的大鼠移植体存活。移植体的存活与自残行为的抑制相关。这表明髓质嗜铬细胞在处理来自受损神经输入的脊髓节段作为抗伤害性药物的局部、持久来源发挥作用。
