新生大鼠5,7 - 二氢麦角隐亭损伤可上调大鼠脊髓中[³H]美舒麦角林标记的5 - HT1C结合位点。

Neonatal 5,7-DHT lesions upregulate [3H]mesulergine-labelled spinal 5-HT1C binding sites in the rat.

作者信息

Pranzatelli M R

机构信息

Department of Neurology, College of Physicians and Surgeons, Columbia University, New York, NY.

出版信息

Brain Res Bull. 1990 Jul;25(1):151-3. doi: 10.1016/0361-9230(90)90266-3.

DOI:10.1016/0361-9230(90)90266-3

PMID:2207701

Abstract

To delineate the involvement of spinal 5-HT1C receptors in supersensitivity and recovery following neonatal 5,7-DHT lesions, we injected rats on postnatal days 2 and 5 with 5,7-DHT or vehicle by intraperitoneal (IP) or intracisternal (IC) injection. [3H]Mesulergine-labelled sites measured 4 or 14 weeks later exhibited a significant increase (+35% for IP and 27% for IC) in Bmax without changes in Kd or nH. Spinal 5-HT content was significantly reduced (-80 to 89%) by either route of 5,7-DHT injection. These data describe novel upregulation of spinal 5-HT1C receptors in rats with neonatal 5,7-DHT lesions. Spinal 5-HT1C receptor upregulation may contribute to the behavioral supersensitivity to L-5-hydroxytryptophan (L-5-HTP) in rats with 5,7-DHT lesions. It does not explain the behavioral recovery we found previously only after IP 5,7-DHT injection.

摘要

为了阐明脊髓5-羟色胺1C（5-HT1C）受体在新生大鼠5,7-二氢麦角隐亭（5,7-DHT）损伤后的超敏反应和恢复过程中的作用，我们在出生后第2天和第5天通过腹腔内（IP）或脑池内（IC）注射的方式给大鼠注射5,7-DHT或赋形剂。在4周或14周后测量的[3H]美舒麦角标记位点显示，最大结合容量（Bmax）显著增加（IP注射增加35%，IC注射增加27%），而解离常数（Kd）或Hill系数（nH）没有变化。通过任何一种5,7-DHT注射途径，脊髓5-羟色胺（5-HT）含量均显著降低（-80%至89%）。这些数据描述了新生大鼠5,7-DHT损伤后脊髓5-HT1C受体的新型上调。脊髓5-HT1C受体上调可能导致5,7-DHT损伤大鼠对L-5-羟色氨酸（L-5-HTP）的行为超敏反应。但这并不能解释我们之前仅在IP注射5,7-DHT后发现的行为恢复现象。