VNS Institute of Pharmacy, Neelbud, Bhopal, Madhya Pradesh, India.
Pharm Biol. 2011 Dec;49(12):1306-10. doi: 10.3109/13880209.2011.596210.
The papaya is the fruit of the plant Carica papaya L. (Caricaceae) used in India. Fruit and latex are both rich in an enzyme called papain. It is used as a folk remedy for contraception and abortion.
The present study explored the anti-hyperlipidemic effect of the ether- and water-soluble fractions of C. papaya ethanol extract in olive oil-induced hyperlipidemic rats. The study also involved chromatographic studies of extract and fractions.
Flash chromatography was done for the most active fraction. The extract and fractions were administered orally at doses of 200 and 400 mg/kg body weight in rats. Olive oil (5 mL/kg oral dose) was administered 30 min after treatment. Blood was collected and centrifuged at 3000 rpm for 15-20 min and subjected to biochemical analysis.
The study dose-dependently inhibited the total cholesterol (TC), triglycerides (TG), low-density lipoproteins (LDL) level, and significantly increased high-density lipoprotein (HDL) level. Phytochemical screening revealed the presence of fats in the ether fraction, whereas the water fraction revealed the presence of tannins, alkaloids, glycosides. UV λ(max) was found to be 217 nm with a melting point of 41°C for the isolated component.
The anti-hyperlipidemic effect was evaluated in olive oil-loaded rats. Acute treatment caused stimulatory effect on HDL level and inhibition in TC and TG elevation induced by olive oil. The extract and water fraction showed protective action by increasing the HDL cholesterol level.
木瓜是番木瓜属植物 Carica papaya L.（番木瓜科）的果实，在印度被使用。果实和乳汁都富含一种名为木瓜蛋白酶的酶。它被用作民间避孕和堕胎的补救措施。
本研究探讨了番木瓜乙醇提取物的乙醚和水溶部分在橄榄油诱导的高脂血症大鼠中的抗高血脂作用。还进行了提取物和馏分的色谱研究。
对最活跃的馏分进行闪式色谱分离。提取物和馏分以 200 和 400mg/kg 体重的剂量口服给予大鼠。橄榄油（5ml/kg 口服剂量）在治疗后 30 分钟给予。收集血液并以 3000rpm 离心 15-20 分钟，进行生化分析。
该研究剂量依赖性地抑制总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL）水平，并显著增加高密度脂蛋白（HDL）水平。植物化学筛选显示乙醚部分存在脂肪，而水部分则存在单宁、生物碱、糖苷。分离成分的 UV λ(max) 为 217nm，熔点为 41°C。
在橄榄油负荷大鼠中评估了抗高血脂作用。急性治疗对 HDL 水平有刺激作用，并抑制橄榄油引起的 TC 和 TG 升高。提取物和水馏分通过增加 HDL 胆固醇水平显示出保护作用。
