State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, P.R. China.
J Ocul Pharmacol Ther. 2012 Apr;28(2):186-93. doi: 10.1089/jop.2011.0136. Epub 2011 Nov 11.
To evaluate the prophylactic effects of topical gemifloxacin, pazufloxacin, moxifloxacin, and levofloxacin for experimental Staphylococcus aureus endophthalmitis in a rabbit model.
Rabbits were randomly divided into 5 groups with 14 rabbits each. The control group received balanced salt solution (BSS); the other 4 groups received 1 drop of gemifloxacin, pazufloxacin, moxifloxacin, or levofloxacin ophthalmic solution, respectively, in the left eye at each of the following time points: 60, 45, 30, and 15 min before infection, immediately after infection, and then 6, 12, 18, and 24 h postinfection. S. aureus ATCC25923 [5×10(5) colony-forming units (CFUs) in 50 μL] was injected into the aqueous humor of each eye, and the clinical severity of the eyes was assessed 24 h postinfection. Two random eyeballs from each group were collected for histopathologic observation. Aqueous and vitreous samples of 12 other eyes were collected, diluted, and plated to determine the number of recovered CFUs.
Fluoroquinolone-treated eyes had significantly lower clinical scores and lower numbers of bacteria recovered from the aqueous humor than the BSS control group eyes. There was no difference, however, among the 4 fluoroquinolone-treated groups. In contrast, none of the fluoroquinolones reduced the number of bacteria recovered (CFUs) from the vitreous humor. In terms of the histopathologic results, less severe inflammation and relatively well-preserved ocular architecture were observed in fluoroquinolone-treated eyes, when compared with the control group.
Gemifloxacin and pazufloxacin may be as effective as moxifloxacin and levofloxacin for topical prophylaxis and for the treatment of S. aureus-induced endophthalmitis in the rabbit model.
在兔眼金黄色葡萄球菌内眼感染模型中评价局部应用吉米沙星、帕珠沙星、莫西沙星和左氧氟沙星的预防作用。
将兔子随机分为 5 组，每组 14 只。对照组给予平衡盐溶液（BSS）；其余 4 组分别于感染前 60、45、30 和 15 分钟以及感染后即刻、6、12、18 和 24 小时，左眼各滴 1 滴吉米沙星、帕珠沙星、莫西沙星或左氧氟沙星滴眼液。将金黄色葡萄球菌 ATCC25923（5×10（5）个 CFU 于 50μL 中）注入每只眼的房水中，于感染后 24 小时评估眼部的临床严重程度。每组随机取 2 只眼球进行组织病理学观察。收集其余 12 只眼的房水和玻璃体液样本，稀释后进行平板培养以确定回收 CFU 的数量。
与 BSS 对照组相比，氟喹诺酮滴眼液治疗的眼睛临床评分明显较低，房水中回收的细菌数量也较少。然而，4 种氟喹诺酮滴眼液治疗组之间没有差异。相比之下，氟喹诺酮类药物均未能减少玻璃体液中回收的细菌数量（CFU）。在组织病理学结果方面，与对照组相比，氟喹诺酮滴眼液治疗的眼睛炎症程度较轻，眼部结构保存相对较好。
吉米沙星和帕珠沙星可能与莫西沙星和左氧氟沙星一样有效，可用于金黄色葡萄球菌性内眼感染的局部预防和治疗。
