Reisner A H, Bucholtz C A, Bell G A, Tsui K, Rosenfeld D, Herman G T
CSIRO, Division of Biotechnology, Laboratory for Molecular Biology, North Ryde, NSW, Australia.
Comput Appl Biosci. 1990 Jul;6(3):253-61. doi: 10.1093/bioinformatics/6.3.253.
A complete system has been developed to utilize histological serial sections for two- and three-dimensional image reconstructions. Eighty to 120 sections are digitized using a personal computing system augmented with a imaging board and CCD camera. The image files are transmitted to a VAX computer for processing and image reconstruction, and the processed images are transmitted back to the personal computer for display and recording using a film recorder or PostScript printer. The software developed for the system allows serial sections to be placed into proper registration in a 256(3) array, 256 grey levels. Autoradiographs of the sections are obtained in the presence of appropriate standards which are used to recalibrate grey levels to represent linearly the radioactivity of each pixel in the sections and scale the values to allow maximum use of the grey scale. Starting from coronally sectioned material the system has been used to analyse and reconstruct rat nasal turbinates. In two dimensions horizontal and sagittal sections have been obtained while in three dimensions back-to-front and surface-rendered images have been constructed. Useful rendering of differential metabolic activity within an organ of complex geometry has been obtained, and there appears to be no reason why the system cannot be used for any material for which serial sectioning is appropriate.
已开发出一套完整的系统，用于利用组织学连续切片进行二维和三维图像重建。使用配备成像板和电荷耦合器件（CCD）相机的个人计算机系统对80至120张切片进行数字化处理。图像文件被传输到VAX计算机进行处理和图像重建，处理后的图像再传输回个人计算机，使用胶片记录器或PostScript打印机进行显示和记录。为该系统开发的软件允许将连续切片在256(3)阵列（256个灰度级）中进行正确配准。在存在适当标准的情况下获取切片的放射自显影片，这些标准用于重新校准灰度级，以线性表示切片中每个像素的放射性，并对数值进行缩放，以便最大程度地利用灰度范围。从冠状切片材料开始，该系统已用于分析和重建大鼠鼻甲。在二维方面，已获得水平和矢状切片，而在三维方面，已构建从前到后的图像和表面渲染图像。已获得复杂几何形状器官内差异代谢活性的有用渲染结果，而且似乎没有理由该系统不能用于任何适合进行连续切片的材料。
