结合剂量和注射量以提高特定放射性药物用于前哨淋巴结检测的性能。

Combining dose and injection volume for good performance of a specific radiopharmaceutical for sentinel node detection.

机构信息

Radiopharmacy Center, Institute of Energetic and Nuclear Research, Sao Paulo, SP 05508-000, Brazil.

出版信息

Nucl Med Biol. 2012 Jan;39(1):145-53. doi: 10.1016/j.nucmedbio.2011.07.004. Epub 2011 Nov 12.

DOI:10.1016/j.nucmedbio.2011.07.004

PMID:22079036

Abstract

INTRODUCTION

The aim of this work was to quantify the effects of injection volume at different technetium-99m specific radiotracer doses on its lymphatic movement in animal model.

PROCEDURES

Effects of injection volume (50, 100 μl) at different doses (0.05, 0.135, 0.22 nmol) on popliteal node (PN) detection were studied in rats. The radiotracer under study was (99m)Technetium-cysteine-mannose-dextran conjugate (30 kDa).

RESULTS

At 0.05 nmol dose, higher PN uptake was observed at 50 μl injection volume (2.6 fold increase). Conversely, at 0.135 nmol dose, an increase of radiotracer retention in PN was achieved at 100 μl volume, 78% higher than 50 μl. However, at 0.22 nmol dose, the injection volume changes did not influence on the PN uptake. Considering as suitable radiotracer performance: high PN uptake and extraction, better combinations were 0.05 nmol/50 μl, 0.135 nmol/100 μl, 0.22/50 μl.

CONCLUSION

Suitable performances could be reached by proper combinations of dose, injection volume and concentration for a specific radiotracer used in sentinel lymph node detection.

摘要

简介

本研究旨在量化在不同锝-99m 特异放射性示踪剂剂量下注射体积对其在动物模型中淋巴运动的影响。

方法

在大鼠中研究了不同剂量（0.05、0.135、0.22 nmol）下注射体积（50、100 μl）对隐窝淋巴结（PN）检测的影响。所研究的放射性示踪剂为（99m）锝-半胱氨酸-甘露糖-葡聚糖缀合物（30 kDa）。

结果

在 0.05 nmol 剂量下，50 μl 注射体积观察到更高的 PN 摄取（增加 2.6 倍）。相反，在 0.135 nmol 剂量下，100 μl 体积可增加放射性示踪剂在 PN 中的保留，比 50 μl 高 78%。然而，在 0.22 nmol 剂量下，PN 摄取的注射体积变化没有影响。考虑到合适的放射性示踪剂性能：高 PN 摄取和提取，更好的组合是 0.05 nmol/50 μl、0.135 nmol/100 μl、0.22/50 μl。