积雪草苷对MCF-7细胞摄取99mTc-替曲膦和99mTc-甲氧基异丁基异腈的影响。

Effect of asiaticoside on 99mTc-tetrofosmin and 99mTc-sestamibi uptake in MCF-7 cells.

作者信息

Al-Saeedi Fatma J, Bitar Milad, Pariyani Smitha

机构信息

Department of Nuclear Medicine, Faculty of Medicine, Kuwait University, Safat, Kuwait.

出版信息

J Nucl Med Technol. 2011 Dec;39(4):279-83. doi: 10.2967/jnmt.111.091868. Epub 2011 Nov 11.

DOI:10.2967/jnmt.111.091868

PMID:22080437

Abstract

UNLABELLED

This study was done to examine the effect of asiaticoside on MCF-7 cell uptake of (99m)Tc-tetrofosmin ((99m)Tc-Tfos) and (99m)Tc-sestamibi ((99m)Tc-MIBI).

METHODS

The 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the effect of a 50% inhibitory concentration of asiaticoside on MCF-7 cell proliferation. MCF-7 cells were treated with 10, 20, 30, 40, and 50 μM asiaticoside for 48 h and then incubated with 59.2 MBq of either (99m)Tc-Tfos or (99m)Tc-MIBI tracer for 60 min. The uptake of the tracers was measured with a dose calibrator.

RESULTS

The 50% inhibitory concentration of asiaticoside for MCF-7 cells was determined with the MTT assay to be 40 μM. The uptake results were expressed as the mean ± SE radioactivity in MBq/mg of protein, and P values were also calculated (P values of 0.03 indicated significant differences). In the control (no asiaticoside) and at 10, 20, 30, 40, and 50 μM asiaticoside, the mean levels of (99m)Tc-Tfos uptake were 0.79 (SE, 0.059) (P = 0.14), 0.84 (SE, 0.057) (P = 0.60), 0.47 (SE, 0.034) (P = 0.03), 0.40 (SE, 0.050) (P = 0.03), 0.37 (SE, 0.050) (P = 0.03), and 0.15 (SE, 0.023) (P = 0.03), respectively; the mean levels of (99m)Tc-MIBI uptake were 0.95 (SE, 0.007) (P = 0.14), 0.81 (SE, 0.009) (P = 0.60), 0.79 (SE, 0.019) (P = 0.03), 0.63 (SE, 0.004) (P = 0.03), 0.13 (SE, 0.006) (P = 0.03), and 0.07 (SE, 0.008) (P = 0.03), respectively. Asiaticoside concentrations of 10, 20, 30, 40, and 50 μM revealed the uptake kinetics for both (99m)Tc-Tfos and (99m)Tc-MIBI in MCF-7 cells. (99m)Tc-Tfos and (99m)Tc-MIBI showed similar trends; the radioactivity uptake was dose dependent, and asiaticoside inhibited 16% and 47% of (99m)Tc-Tfos uptake and (99m)Tc-MIBI uptake in MCF-7 cells, respectively.

CONCLUSION

This study showed that asiaticoside, acting as a biochemical modulator, may induce apoptosis and enhance antitumor activity in MCF-7 cells, as determined by (99m)Tc-Tfos and (99m)Tc-MIBI uptake. These findings are promising for cancer chemotherapy. Future studies should be performed to confirm our findings and to further delineate the clinical role of asiaticoside.

摘要

未标记

本研究旨在检测积雪草苷对MCF-7细胞摄取（99m）锝-替曲膦（（99m）Tc-Tfos）和（99m）锝-甲氧基异丁基异腈（（99m）Tc-MIBI）的影响。

方法

采用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）法评估积雪草苷50%抑制浓度对MCF-7细胞增殖的影响。MCF-7细胞用10、20、30、40和50μM积雪草苷处理48小时，然后与59.2MBq的（99m）Tc-Tfos或（99m）Tc-MIBI示踪剂孵育60分钟。用剂量校准仪测量示踪剂的摄取量。

结果

通过MTT法测定积雪草苷对MCF-7细胞的50%抑制浓度为40μM。摄取结果以每毫克蛋白质中放射性活度的平均值±标准误表示，同时也计算了P值（P值为0.03表示有显著差异）。在对照组（无积雪草苷）以及10、20、30、40和50μM积雪草苷处理组中，（99m）Tc-Tfos摄取的平均水平分别为0.79（标准误，0.059）（P = 0.14）、0.84（标准误，0.057）（P = 0.60）、0.47（标准误，0.034）（P = 0.03）、0.40（标准误，0.050）（P = 0.03）、0.37（标准误，0.050）（P = 0.03）和0.15（标准误，0.023）（P = 0.03）；（99m）Tc-MIBI摄取的平均水平分别为0.95（标准误，0.007）（P = 0.14）、0.81（标准误，0.009）（P = 0.60）、0.79（标准误，0.019）（P = 0.03）、0.63（标准误，0.004）（P = 0.03）、0.13（标准误，0.006）（P = 0.03）和0.07（标准误，0.008）（P = 0.03）。10、20、30、40和50μM的积雪草苷浓度揭示了MCF-7细胞中（99m）Tc-Tfos和（99m）Tc-MIBI的摄取动力学。（99m）Tc-Tfos和（99m）Tc-MIBI呈现相似趋势；放射性摄取呈剂量依赖性，积雪草苷分别抑制MCF-7细胞中（99m）Tc-Tfos摄取和（99m）Tc-MIBI摄取的16%和47%。