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转化生长因子-β1在补充了表皮生长因子（EGF）和胰岛素的无血清软琼脂培养系统中刺激莫里斯肝癌细胞的增殖。

Transforming growth factor-beta 1 stimulates proliferation of Morris hepatoma cells in serum-free soft agar culture system supplemented with EGF and insulin.

作者信息

Guzik K, Klein A

机构信息

Institute of Molecular Biology, Jagiellonian University, Krakow, Poland.

出版信息

Cancer Lett. 1990 Oct 8;54(1-2):51-6. doi: 10.1016/0304-3835(90)90090-k.

DOI:10.1016/0304-3835(90)90090-k
PMID:2208089
Abstract

The anchorage-independent growth of Morris hepatoma 7777 (MH) cells in serum-free medium was examined. The influence of insulin, epidermal growth factor and transforming growth factor beta 1 (TGF-beta 1) on [3H]thymidine incorporation and colony formation of the investigated cells was described. Contrary to normal rat hepatocytes TGF-beta 1 plus EGF and insulin were found to stimulate MH cells proliferation in presented conditions. A simple, chemically-defined culture system suitable for research on mitogenic peptides was proposed.

摘要

检测了Morris肝癌7777（MH）细胞在无血清培养基中的非锚定依赖性生长。描述了胰岛素、表皮生长因子和转化生长因子β1（TGF-β1）对所研究细胞的[3H]胸腺嘧啶核苷掺入和集落形成的影响。与正常大鼠肝细胞相反，发现TGF-β1加表皮生长因子和胰岛素在所述条件下刺激MH细胞增殖。提出了一种适用于有丝分裂原性肽研究的简单、化学成分明确的培养系统。

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