State Key Laboratory of Animal Nutrition, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, PR China.
Meat Sci. 2012 Mar;90(3):658-64. doi: 10.1016/j.meatsci.2011.10.010. Epub 2011 Oct 28.
The aim of this study was to develop near-infrared reflectance spectroscopy (NIRS) calibrations for determination of the fatty acids (FA) in broiler breast meat. A total of 144 breast meat samples were freeze-dried and divided into calibration set and validation set. Calibration models were developed for FA including C14:0, C16:0, C16:1n-7, C18:0, C18:1n-7, C18:1n-9, C18:2n-6, C18:3n-3, C18:3n-6, C20:0, C20:1n-9, C20:2n-6, C20:4n-6, C20:5n-3, C22:4n-6, C22:6n-3, C24:0 and C24:1n-9. Calibration models for FA groups were also developed. Calibrations based on the absolute FA content were more accurate than those based on the relative composition (%). The coefficients of determination of FA and FA groups (based on the absolute content) except C18:3n-6, C20:0, C20:2n-6 and C24:1n-9, were between 0.86 and 0.98 for calibration, and 0.83 and 0.97 for validation. The results indicate NIRS can be a feasible and rapid method for determination of FA with a mean concentration over 0.10g/kg.
本研究旨在开发近红外反射光谱（NIRS）校准方法，以测定肉鸡胸脯肉中的脂肪酸（FA）。共采集了 144 个胸脯肉样本，经冷冻干燥后分为校准集和验证集。建立了包括 C14:0、C16:0、C16:1n-7、C18:0、C18:1n-7、C18:1n-9、C18:2n-6、C18:3n-3、C18:3n-6、C20:0、C20:1n-9、C20:2n-6、C20:4n-6、C20:5n-3、C22:4n-6、C22:6n-3、C24:0 和 C24:1n-9 的 FA 校准模型。还建立了 FA 组的校准模型。基于 FA 绝对含量的校准比基于相对组成（%）的校准更准确。除 C18:3n-6、C20:0、C20:2n-6 和 C24:1n-9 外，FA 和 FA 组（基于绝对含量）的决定系数（r2）在 0.86 到 0.98 之间，验证集的 r2 在 0.83 到 0.97 之间。结果表明，NIRS 可以成为一种可行且快速的方法，用于测定平均浓度超过 0.10g/kg 的 FA。
