Alomone Labs Ltd., P.O. Box 4287, Jerusalem, 91042, Israel,
Purinergic Signal. 2012 Feb;8(Suppl 1):61-79. doi: 10.1007/s11302-011-9278-z. Epub 2011 Nov 16.
The broad expression pattern of the G protein-coupled P2Y receptors has demonstrated that these receptors are fundamental determinants in many physiological responses, including neuromodulation, vasodilation, inflammation, and cell migration. P2Y receptors couple either G(q) or G(i) upon activation, thereby activating different signaling pathways. Ionotropic ATP (P2X) receptors bind extracellular nucleotides, a signal which is transduced within the P2X protein complex into a cation channel opening, which usually leads to intracellular calcium concentration elevation. As such, this family of proteins initiates or shapes several cellular processes including synaptic transmission, gene expression, proliferation, migration, and apoptosis. The ever-growing range of applications for antibodies in the last 30 years attests to their major role in medicine and biological research. Antibodies have been used as therapeutic tools in cancer and inflammatory diseases, as diagnostic reagents (flow cytometry, ELISA, and immunohistochemistry, to name a few applications), and in widespread use in biological research, including Western blot, immunoprecipitation, and ELISPOT. In this article, we will showcase several of the advances that scientists around the world have achieved using the line of antibodies developed at Alomone Labs for P2Y and P2X receptors.
G 蛋白偶联 P2Y 受体的广泛表达模式表明，这些受体是许多生理反应的基本决定因素，包括神经调节、血管舒张、炎症和细胞迁移。P2Y 受体在激活时与 G(q)或 G(i)偶联，从而激活不同的信号通路。离子型 ATP（P2X）受体结合细胞外核苷酸，该信号在 P2X 蛋白复合物中被转导为阳离子通道开放，通常导致细胞内钙离子浓度升高。因此，这一家族的蛋白质启动或塑造了几种细胞过程，包括突触传递、基因表达、增殖、迁移和细胞凋亡。在过去 30 年中，抗体在各个领域的应用范围不断扩大，证明了它们在医学和生物学研究中的重要作用。抗体已被用作癌症和炎症性疾病的治疗工具，作为诊断试剂（流式细胞术、ELISA 和免疫组织化学，仅举几例应用），并广泛用于生物学研究，包括 Western blot、免疫沉淀和 ELISPOT。在本文中，我们将展示世界各地的科学家使用 Alomone 实验室开发的针对 P2Y 和 P2X 受体的抗体系列取得的一些进展。
