Service de Bactériologie-Virologie, INSERM U914 "Emerging Resistance to Antibiotics", Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine and Université Paris-Sud, Kremlin-Bicêtre, France.
FEMS Microbiol Lett. 2011 Nov;324(2):111-6. doi: 10.1111/j.1574-6968.2011.02392.x. Epub 2011 Oct 3.
The latest threat of multidrug-resistant Gram-negative bacteria corresponds to the emergence of carbapenemase NDM-1 (New Delhi metallo-β-lactamase) producers, mostly in Enterobacteriacae. Five bla(NDM) (-1) -positive plasmids of different incompatibility groups (IncL/M, FII, A/C and two untypeable plasmids) from clinical Enterobacteriaceae were evaluated for conjugation properties and host specificity. Successful conjugative transfers were obtained using all tested enterobacterial species as recipients (Escherichia coli, Klebsiella pneumoniae, Salmonella typhimurium and Proteus mirabilis) and all plasmid types. Conjugation frequencies varied from 1 × 10(-4) to 6 × 10(-8) transconjugants per donor. Higher conjugation rates were obtained for two plasmids at 30 °C compared with that observed at 25 and 37 °C. Carbapenems used as selector did not lead to higher conjugation frequencies. None of the five plasmids was transferable to Acinetobacter baumannii or Pseudomonas aeruginosa by conjugation. This work underlines how efficient the spread of the carbapenemase bla(NDM) (-1) gene could be among Enterobacteriaceae.
最近出现的耐多药革兰氏阴性菌的威胁与碳青霉烯酶 NDM-1（新德里金属β-内酰胺酶）的产生有关，这些产酶菌主要存在于肠杆菌科中。从临床肠杆菌科中评估了 5 种不同不相容群（IncL/M、FII、A/C 和 2 种未定型质粒）的 bla（NDM）（-1）阳性质粒的接合特性和宿主特异性。使用所有测试的肠杆菌作为受体（大肠杆菌、肺炎克雷伯菌、鼠伤寒沙门氏菌和奇异变形杆菌）和所有质粒类型，均成功获得了接合转移。接合频率从每供体 1×10（-4）到 6×10（-8）转导子不等。与在 25 和 37°C 时相比，两种质粒在 30°C 时的接合率更高。作为选择剂的碳青霉烯类药物并没有导致更高的接合频率。通过接合，这 5 种质粒均不能转移到鲍曼不动杆菌或铜绿假单胞菌中。这项工作强调了碳青霉烯酶 bla（NDM）（-1）基因在肠杆菌科中的传播效率有多高。
