Liu Hui, Chang Nai-bai, Pei Lei, Ning Shang-yong, Li Jiang-tao, Xing Bao-li, Xu Xiao-dong
Department of Hematology, Beijing Hospital, Beijing 100730, China.
Zhonghua Nei Ke Za Zhi. 2011 Aug;50(8):683-6.
To explore the cytogenetic characteristics of acute myeloid leukemia (AML) patients.
The karyotype analysis was performed in 178 AML using the short-term culture of bone marrow cell and G-banding technique.
Among the 178 patients, 171 had enough metaphases for analysis and 128 (74.9%) had clonal karyotypic abnormalities. Twenty-seven patients were secondary to myelodysplastic syndrome (MDS-AML), with 25 (92.6%) patients carrying clonal karyotypic abnormalities. Among the remaining 144 patients of de novo AML, 103 (71.5%) had clonal karyotypic abnormalities. The rate of abnormal clonal karyotype was higher in MDS-AML than that of de novo AML (P = 0.021). Among the 171 patients, 41 (24.0%) were in favorable risk group, 80(46.8%) in intermediate risk group and 50 (29.2%) in adverse risk group. t(15;17) was the most common chromosomal aberration. The majority intermediate risk chromosomal aberration was normal karyotype. The most common cytogenetic abnormality among adverse group was a complex karyotype. Adverse cytogenetic aberrations, such as -5/5q-, -7/7q-, frequently occurred in conjunction with one another as part of a complex karyotype. Totally 75 patients were 60 years or older, among them, 16.0% were in favorable risk group, 48.0% in intermediate risk group and 36.0% in adverse risk group. Among 96 younger patients, 30.2% were in favorable risk group, 45.8% in intermediate risk group and 24.0% in adverse risk group. The rate of favorable risk chromosomal aberration was lower in elder patients than in younger (P = 0.031). The rate of adverse risk chromosomal aberration and the rate of monosomal karyotype were higher in MDS-AML than in de novo AML patients (P < 0.001).
The most common favorable, intermediate and adverse chromosomal aberrations were t(15;17), normal karyotype and complex karyotype respectively. The karyotype was poor in MDS-AML and elder AML patients.
探讨急性髓系白血病（AML）患者的细胞遗传学特征。
采用骨髓细胞短期培养及G显带技术对178例AML患者进行核型分析。
178例患者中，171例有足够的中期分裂相可供分析，128例（74.9%）有克隆性核型异常。27例患者继发于骨髓增生异常综合征（MDS-AML），其中25例（92.6%）有克隆性核型异常。在其余144例初发AML患者中，103例（71.5%）有克隆性核型异常。MDS-AML患者克隆性核型异常率高于初发AML患者（P = 0.021）。171例患者中，41例（24.0%）为低危组，80例（46.8%）为中危组，50例（29.2%）为高危组。t(15;17)是最常见的染色体畸变。大多数中危组染色体畸变核型正常。高危组最常见的细胞遗传学异常是复杂核型。-5/5q-、-7/7q-等不良细胞遗传学畸变常作为复杂核型的一部分同时出现。共有75例患者年龄在60岁及以上，其中低危组占16.0%，中危组占48.0%，高危组占36.0%。在96例年轻患者中，低危组占30.2%，中危组占45.8%，高危组占24.0%。老年患者低危组染色体畸变率低于年轻患者（P = 0.031）。MDS-AML患者高危组染色体畸变率和单倍体核型率高于初发AML患者（P < 0.001）。
最常见的低危、中危和高危染色体畸变分别为t(15;17)、核型正常和复杂核型。MDS-AML患者和老年AML患者核型较差。
