Prince Felipe Research Centre, Valencia, Spain.
FEMS Yeast Res. 2011 Dec;11(8):656-9. doi: 10.1111/j.1567-1364.2011.00754.x. Epub 2011 Sep 27.
In Saccharomyces cerevisiae, the cell integrity pathway plays a role in the oxidative stress response. In this study, we show that the Pkc1 protein mediates oxidative signalling by helping to downregulate ribosomal gene expression when cells are exposed to hydrogen peroxide. An active actin cytoskeleton is required for this function, because the cells blocked in actin polymerisation were unable to repress ribosomal gene transcription. Following the invertase secretion pattern, we hypothesize that oxidative stress induced by hydrogen peroxide could have affected the latter steps of secretion. This would explain why the Pkc1 function was required to repress ribosomal biogenesis.
在酿酒酵母中，细胞完整性途径在氧化应激反应中发挥作用。在这项研究中，我们表明 Pkc1 蛋白通过帮助下调细胞暴露于过氧化氢时的核糖体基因表达来介导氧化信号。当细胞被阻断在肌动蛋白聚合时，活性肌动蛋白细胞骨架是这一功能所必需的，因为这些细胞无法抑制核糖体基因转录。根据转化酶分泌模式，我们假设过氧化氢引起的氧化应激可能影响了分泌的后几个步骤。这就解释了为什么需要 Pkc1 功能来抑制核糖体生物发生。
