[白细胞介素-8基因多态性与中国炎症性肠病患者的相关性]

[Association of IL-8 gene polymorphisms with inflammatory bowel disease in Chinese patients].

作者信息

Liang Wan-dong, Li Jin-song, Li Ke-shen, Jin Jie, Yan Jun, Yang Ji-feng, Bi Yun-tian

机构信息

School of Life Sciences, Wenzhou Medical College, Zhejiang, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2011 Jul 12;91(26):1825-9.

PMID:22093783

Abstract

OBJECTIVE

To investigate the association of interleukin 8 (IL-8) gene polymorphisms with the risks of inflammatory bowel disease (IBD).

METHODS

Single nucleotide polymorphisms (SNPs) of IL-8 gene at -845 T/C, -738 T/A, -353 A/T, -251 T/A and +678 T/C were analyzed in 183 IBD patients. They included Crohn's disease (CD, n = 41), ulcerative colitis (UC, n = 142) and healthy controls (n = 160). The methods of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and polymerase chain reaction-sequence specific primers (PCR-SSP) were employed.

RESULTS

No association was observed between any of these five SNPs in IL-8 gene with the occurrence of IBD. A specific haplotype AAT (-353 A/T, -251 T/A & +678 T/C) was over-represented in UC cases when compared with controls (31.0% vs 23.7%, P = 0.046). But the distributions of this haplotype did not show significant difference between CD cases and controls.

CONCLUSION

Our data support a significant but modest association between the AAT haplotype of IL-8 gene and UC (OR = 1.441, 95%CI 1.007 - 2.063).

摘要

目的

探讨白细胞介素8（IL-8）基因多态性与炎症性肠病（IBD）发病风险的相关性。

方法

对183例IBD患者进行IL-8基因-845 T/C、-738 T/A、-353 A/T、-251 T/A和+678 T/C位点的单核苷酸多态性（SNP）分析。患者包括克罗恩病（CD，n = 41）、溃疡性结肠炎（UC，n = 142）及健康对照者（n = 160）。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）及聚合酶链反应-序列特异性引物（PCR-SSP）方法。