Sun Xiao-le, Yang Zi, Wang Jia-lue, Sun Min-na, Wu Shu-ying, Wang Xiao-ye
Department of Obstetrics & Gynecology, Peking University Third Hospital, Beijing, China.
Zhonghua Yi Xue Za Zhi. 2011 Aug 9;91(29):2026-9.
To explore the correlation between severe preeclampsia and abnormal expression of long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD).
Serum-free trophoblast cells cultured in vitro were divided into 4 groups under the stimulations of normal pregnancy serum (NP group), early onset severe preeclampsia serum (E-PE group), late onset severe preeclampsia serum (L-PE group) and HELLP (hemolysis, elevated liver enzymes & low platelets) syndrome serum (HELLP group) respectively. The expressions of mRNA and protein of LCHAD in trophoblast cells were detected by real-time polymerase chain reaction (PCR) and Western blot.
(1) Expression of LCHAD mRNA: the relative expressions of mRNA of LCHAD in NP, E-PE, L-PE and HELLP groups were 1.00 ± 0.00, 3.08 ± 0.22, 1.62 ± 0.23 and 3.36 ± 0.18 respectively. The relative expressions of LCHAD mRNA were significantly reduced in the E-PE, L-PE and HELLP groups versus the NP group (P < 0.05). Compared with the L-PE group, the gene expressions of LCHAD significantly decreased in the E-PE and HELLP groups (P < 0.05) while no significant difference was found between the E-PE and HELLP groups (P > 0.05). (2) Expression of LCHAD protein: the relative expressions of LCHAD protein were 4.94 ± 0.02, 2.93 ± 0.13, 4.14 ± 0.06 and 2.80 ± 0.09 in the NP, E-PE, L-PE and HELLP groups respectively. The protein expressions of LCHAD were remarkably reduced in the E-PE, L-PE and HELLP groups versus the NP group (P < 0.05). The expressions of LCHAD protein remarkably decreased in the E-PE and HELLP groups versus the L-PE group (P < 0.05) while no significant difference was found between the E-PE and HELLP groups (P > 0.05).
Long chain fatty acid oxidation is involved in the pathogenesis and development of preeclampsia. The expressions of LCHAD gene and protein are remarkably affected by early onset severe preeclampsia and HELLP syndrome. The interacting mechanism and influence between fatty acid oxidation and the development of preeclampsia are worth further exploring.
探讨重度子痫前期与长链3-羟基酰基辅酶A脱氢酶（LCHAD）异常表达之间的相关性。
体外培养的无血清滋养层细胞分别在正常妊娠血清（NP组）、早发型重度子痫前期血清（E-PE组）、晚发型重度子痫前期血清（L-PE组）和HELLP（溶血、肝酶升高及血小板减少）综合征血清（HELLP组）刺激下分为4组。采用实时聚合酶链反应（PCR）和蛋白质免疫印迹法检测滋养层细胞中LCHAD的mRNA和蛋白表达。
（1）LCHAD mRNA表达：NP组、E-PE组、L-PE组和HELLP组中LCHAD mRNA的相对表达量分别为1.00±0.00、3.08±0.22、1.62±0.23和3.36±0.18。与NP组相比，E-PE组、L-PE组和HELLP组中LCHAD mRNA的相对表达量显著降低（P<0.05）。与L-PE组相比，E-PE组和HELLP组中LCHAD的基因表达显著降低（P<0.05），而E-PE组和HELLP组之间无显著差异（P>0.05）。（2）LCHAD蛋白表达：NP组、E-PE组、L-PE组和HELLP组中LCHAD蛋白的相对表达量分别为4.94±0.02、2.93±0.13、4.14±0.06和2.80±0.09。与NP组相比，E-PE组、L-PE组和HELLP组中LCHAD的蛋白表达显著降低（P<0.05）。与L-PE组相比，E-PE组和HELLP组中LCHAD蛋白的表达显著降低（P<0.05），而E-PE组和HELLP组之间无显著差异（P>0.05）。
长链脂肪酸氧化参与子痫前期的发病机制和发展过程。早发型重度子痫前期和HELLP综合征显著影响LCHAD基因和蛋白的表达。脂肪酸氧化与子痫前期发展之间的相互作用机制及影响值得进一步探讨。
