Mochizuki Yuki, Nemoto Naoto
Graduate School of Science and Engineering, Saitama University, Saitama, Japan.
Methods Mol Biol. 2012;805:237-50. doi: 10.1007/978-1-61779-379-0_13.
Protein scaffolds containing some disulfide bonds (e.g., Knottin, Kunitz domain, etc.) are promising candidates for molecular recognition. cDNA display has been developed to screen functional disulfide-rich peptide aptamers from a vast library by promoting disulfide bond shuffling after the synthesis of peptides in a cell-free translation system. Here we present a detailed protocol for the selection of disulfide-rich peptide aptamers against interleukin 6 receptor (IL-6R) from a 35-amino acid peptide library containing 32 amino acids in the random region, which is linked to its genotype by cDNA display.
含有一些二硫键的蛋白质支架（如结蛋白、库尼茨结构域等）是分子识别的有前途的候选物。cDNA展示技术已被开发出来，通过在无细胞翻译系统中合成肽后促进二硫键重排，从庞大的文库中筛选功能性富含二硫键的肽适配体。在此，我们展示了一个详细的方案，用于从一个在随机区域含有32个氨基酸的35氨基酸肽文库中筛选针对白细胞介素6受体（IL-6R）的富含二硫键的肽适配体，该文库通过cDNA展示与它的基因型相连。
