State Key Laboratory of Veterinary Biotechnology, Harbin 150001, China.
Cancer Lett. 2012 Apr 1;317(1):56-64. doi: 10.1016/j.canlet.2011.11.008. Epub 2011 Nov 15.
Cisplatin (DDP) is widely used in lung cancer chemotherapy. However, cisplatin resistance represents a major obstacle in effective clinical treatment. This study aims to investigate whether Newcastle disease virus (NDV) exhibits an oncolytic effect on cisplatin-resistant A549 lung cancer cells. We found that NDV induced A549/DDP cell apoptosis via the caspase pathway, particularly involving caspase-9, while the mitogen-activated protein kinase (MAPK) and Akt pathways also contributed to apoptotic induction. Furthermore, NDV displayed oncolytic effects in a mouse A549/DDP lung cancer model. Collectively, our data indicate that NDV could overcome the cisplatin resistance in lung cancer cells in vitro and in vivo.
顺铂（DDP）广泛应用于肺癌的化疗。然而，顺铂耐药是临床有效治疗的主要障碍。本研究旨在探讨新城疫病毒（NDV）对顺铂耐药 A549 肺癌细胞是否具有溶瘤作用。我们发现，NDV 通过半胱氨酸天冬氨酸蛋白酶（caspase）途径诱导 A549/DDP 细胞凋亡，特别是 caspase-9 途径，而丝裂原活化蛋白激酶（MAPK）和 Akt 途径也参与了凋亡诱导。此外，NDV 在 A549/DDP 荷瘤小鼠模型中也表现出溶瘤作用。综上所述，我们的数据表明，NDV 可在体外和体内克服肺癌细胞的顺铂耐药性。
