Miladi-Abdennadher Imen, Abdelmaksoud-Damak Rania, Ayadi Lobna, Khabir Abdelmajid, Amouri Ali, Frikha Foued, Ellouz Sameh, Frikha Mounir, Sellami-Boudawara Tahia, Mokdad-Gargouri Raja
Unité de Génétique du cancer et Production de protéines thérapeutiques, Centre de Biotechnologie de Sfax, Tunisia.
Appl Immunohistochem Mol Morphol. 2011 Dec;19(6):562-8. doi: 10.1097/PAI.0b013e3182143380.
Alterations in different signaling pathways are involved in initiation and progression of colorectal carcinoma, such as those related to p53, MLH1, p16INK4a, Kras, etc.
This study was conducted with the aim to investigate the expression of p16INK4a and p53 in colorectal cancer (CRC) and evaluated their correlation with major clinicopathologic features and patients' survival.
The expression of p16INK4a and p53 were analyzed by immunohistochemistry on 70 paraffin specimens of CRC.
Positive immunostaining for p16INK4a and p53 was observed in 27 (38.6%) and 53 (80%) cases, respectively. Significant correlation between loss of p16INK4a expression and tumor size was found (P=0.008), whereas overexpression of p16INK4a correlated with favorable prognosis parameters, such as absence of lymph node metastasis (P=0.029) and early stage of CRC (P=0.027). Furthermore, p53 overexpression significantly correlated with distal tumor location (P=0.022) and was related to a better overall survival in the group of patients with distal colon carcinomas (P=0.002). Patients with p16INK4a-positive tumors had a significant longer overall survival time than patients with p16INK4a-negative carcinomas (P=0.033). In addition, Cox regression model showed that overexpression of p16INK4a is an independent factor for prognosis with depth of invasion, p53 accumulation, and coincident abnormal expression of p16INK4a or p53.
Our data suggest that the assessment of both p53 and p16INK4a expression might be helpful in predicting prognosis in patients with colorectal cancer.
不同信号通路的改变参与了结直肠癌的发生和发展，如与p53、MLH1、p16INK4a、Kras等相关的信号通路。
本研究旨在调查p16INK4a和p53在结直肠癌（CRC）中的表达，并评估它们与主要临床病理特征及患者生存的相关性。
采用免疫组织化学方法分析70例CRC石蜡标本中p16INK4a和p53的表达。
p16INK4a和p53免疫阳性染色分别见于27例（38.6%）和53例（80%）。发现p16INK4a表达缺失与肿瘤大小显著相关（P=0.008），而p16INK4a过表达与良好的预后参数相关，如无淋巴结转移（P=0.029）和CRC早期（P=0.027）。此外，p53过表达与肿瘤远端位置显著相关（P=0.022），并且与远端结肠癌患者的总体生存率较高相关（P=0.002）。p16INK4a阳性肿瘤患者的总体生存时间显著长于p16INK4a阴性癌患者（P=0.033）。此外，Cox回归模型显示，p16INK4a过表达是侵袭深度、p53积累以及p16INK4a或p53同时异常表达的独立预后因素。
我们的数据表明，评估p53和p16INK4a的表达可能有助于预测结直肠癌患者的预后。
