通过31P核磁共振波谱法测定微粒体UDP-葡糖醛酸基转移酶和核苷二磷酸酶的核苷酸代谢。
Nucleotide metabolism by microsomal UDP-glucuronyltransferase and nucleoside diphosphatase as determine by 31P nuclear-magnetic-resonance spectroscopy.
作者信息
Finch S A, Slater T F, Stier A
出版信息
Biochem J. 1979 Mar 1;177(3):925-30. doi: 10.1042/bj1770925.
Abstract
31P n.m.r. spectroscopy was used to study the nucleotide kinetics of UDP-glucuronyltransferase and associated reactions in the liver microsomal fraction. The effects of Mg2+ and EDTA on these reactions were investigated qualitatively. It was found that the rabbit microsomal fraction has no nucleoside pyrophosphatase activity, that UDP was immediately hydrolysed and that it was released from the microsomal surface. Reverse glucuronyltransferase could be demonstrated. The results are discussed with reference to functional coupling of UDP-glucuronyltransferase to other enzymes and the effects of Mg2+ and EDTA on the system.
摘要
采用31P核磁共振光谱法研究肝脏微粒体部分中尿苷二磷酸葡萄糖醛酸基转移酶的核苷酸动力学及相关反应。定性研究了Mg2+和乙二胺四乙酸（EDTA）对这些反应的影响。结果发现，兔微粒体部分无核苷焦磷酸酶活性，尿苷二磷酸（UDP）立即被水解并从微粒体表面释放。可以证明存在逆向葡萄糖醛酸基转移酶。结合UDP葡萄糖醛酸基转移酶与其他酶的功能偶联以及Mg2+和EDTA对该系统的影响对结果进行了讨论。
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