NAD⁺ 与结合在猪心乳酸脱氢酶上的丙酮酸之间加合物形成的机制。
The mechanism of adduct formation between NAD+ and pyruvate bound to pig heart lactate dehydrogenase.
作者信息
Wilton D C
出版信息
Biochem J. 1979 Mar 1;177(3):951-7. doi: 10.1042/bj1770951.
Abstract
- The rate of adduct formation between NAD+ and enol-pyruvate at the active site of lactate dehydrogenase is determined by the rate of enolization of pyruvate in solution. 2. The proportion of enol-pyruvate solutions is less than 0.01%. 3. The overall dissociation constant of adduct formation is less than 5 X 10(-8) M for pig heart lactate dehydrogenase at pH 7.0. 4. The unusual kinetics for adduct formation previously observed in the case of rabbit muscle lactate dehydrogenase [Griffin & Criddle (1970) Biochemistry 9, 1195--1205] may be attributed to the concentration of enol-pyruvate in solution being considerably less than the concentration of enzyme.
摘要
- 乳酸脱氢酶活性位点处NAD⁺与烯醇丙酮酸之间加合物形成的速率由溶液中丙酮酸的烯醇化速率决定。2. 烯醇丙酮酸溶液的比例小于0.01%。3. 在pH 7.0时，猪心乳酸脱氢酶加合物形成的总解离常数小于5×10⁻⁸ M。4. 先前在兔肌肉乳酸脱氢酶的情况下观察到的加合物形成的异常动力学[Griffin & Criddle(1970) Biochemistry 9, 1195 - 1205]可能归因于溶液中烯醇丙酮酸的浓度远低于酶的浓度。
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2
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Inhibition of lactate dehydrogenase by high concentrations of pyruvate: The nature and removal of the inhibitor.高浓度丙酮酸对乳酸脱氢酶的抑制作用：抑制剂的性质与去除
FEBS Lett. 1969 Jun;3(5):333-337. doi: 10.1016/0014-5793(69)80171-7.
5
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