通过检测自杀活性对嗜温气单胞菌进行快速推测性表型种鉴定。

Rapid presumptive phenospecies identification of mesophilic aeromonads by testing for suicidal activity.

作者信息

Piersimoni C, Morbiducci V, De Sio G, Scalise G

机构信息

Department of Clinical Microbiology, General Hospital Umberto I-Torrette, Ancona, Italy.

出版信息

Eur J Clin Microbiol Infect Dis. 1990 Aug;9(8):608-10. doi: 10.1007/BF01967217.

DOI:10.1007/BF01967217

PMID:2209629

Abstract

A new test combination for presumptive phenospecies identification of mesophilic aeromonads by testing for suicidal activity in conjunction with esculin hydrolysis and gas production from glucose was evaluated. Of 40 clinical isolates of Aeromonas tested statically at 30 degrees C, seven were identified as Aeromonas hydrophilia (non-suicidal, aerogenic, esculin positive), eight as Aeromonas sobria (suicide variable, mostly aerogenic, esculin negative), and 25 as Aeromonas caviae (suicidal, anaerogenic, esculin positive). The results corresponded exactly to those obtained with the conventional biochemical tests. The test combination was rapid and simple to perform.

摘要

通过结合检测自杀活性、七叶苷水解和葡萄糖产气情况，对一种用于嗜温气单胞菌推定表型种鉴定的新检测组合进行了评估。在30℃静态条件下对40株气单胞菌临床分离株进行检测，其中7株被鉴定为嗜水气单胞菌（非自杀性、产气、七叶苷阳性），8株为温和气单胞菌（自杀性可变、大多产气、七叶苷阴性），25株为豚鼠气单胞菌（自杀性、不产气、七叶苷阳性）。结果与传统生化检测结果完全一致。该检测组合操作快速且简单。