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[使用多种耐药菌对MicroScan Rapid plus系列的临床意义]

[Clinical significance on MicroScan Rapid plus series using various antibiotic-resistant bacteria].

作者信息

Fukutomi Yumiko, Uki Nozomu, Oho Megumi, Sugimati Mitsuhiko, Kusaba Kouji, Nagasawa Zenzo, Nakajima Yukari, Hukuoka Asami, Aoki Yousuke, Miyamoto Hiroshi

机构信息

Department of Division of Infectious Disease/Control and Prevention, Saga University Hospital and Department of Pathology & Microbiology, Faculty of Medicine, Saga University.

出版信息

Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi. 2010;21(2):95-105.

Abstract

MicroScan Rapid plus Neg II Series and MicroScan Rapid plus Pos Series by Siemens Healthcare Diagnostics K.K. are the panels which enable to measure identification and antimicrobial susceptibility testing quickly and we have confirmed that it is useful for detecting drug resistance bacteria. As the identification result of comparing Rapid plus series with the current panel by using 143 strains of various drug resistance bacteria, Gram positive cocci was 87. 7%, glucose fermenter was 100% and glucose non-fermenter was 77.3 in Gram negative bacilli. On the evaluation of antimicrobial susceptibility testing, Rapid plus series, in comparison with the current panel, confirmed the lower tendency of MIC value on some drugs, but it basically presented the good concordance rate. In terms of the reporting time of antimicrobial agent, non-fermenter or MRCNS reported the result as needed after 8 hours and it took a little longer time for the report of antimicrobial agent. On the other hand, 80% or higher of antimicrobial agent on panel was reported for intestinal bacteria in 4.5 hours and for MRSA in 6.5 hours. It enabled to report the testing result on the same day. Due to the results above, Rapid plus series was highly valued on the usability, such as the early detection of drug resistance bacteria and the selection of therapeutic agents.

摘要

西门子医疗诊断株式会社的MicroScan Rapid plus Neg II系列和MicroScan Rapid plus Pos系列是能够快速进行鉴定和抗菌药敏试验的检测板,我们已确认其对检测耐药菌很有用。通过使用143株各种耐药菌,将Rapid plus系列与当前检测板进行比较,革兰氏阳性球菌的鉴定结果为87.7%,葡萄糖发酵菌为100%,革兰氏阴性杆菌中的葡萄糖非发酵菌为77.3%。在抗菌药敏试验评估方面,与当前检测板相比,Rapid plus系列在某些药物上的MIC值有降低趋势,但总体一致性率良好。在抗菌药物报告时间方面,非发酵菌或MRCNS在8小时后按需报告结果,抗菌药物报告时间稍长。另一方面,检测板上80%或更高比例的抗菌药物在4.5小时内报告肠道细菌结果,在6.5小时内报告耐甲氧西林金黄色葡萄球菌结果。它能够在同一天报告检测结果。基于上述结果,Rapid plus系列在耐药菌的早期检测和治疗药物选择等实用性方面受到高度评价。

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