Degiovanni G, Hainaut P, Lahaye T, Weynants P, Boon T
Laboratoire de Chirurgie Expérimentale, Tour de Pathologie, Université de Liège, Belgium.
Eur J Immunol. 1990 Aug;20(8):1865-8. doi: 10.1002/eji.1830200835.
Peripheral blood lymphocytes of a melanoma patient were stimulated in vitro with a permanent cell line derived from the autologous tumor. Stable cytolytic T lymphocyte (CTL) clones were obtained that lysed the melanoma cell line and did not lyse autologous Epstein-Barr virus-transformed B lymphocytes or K-562 cells. These CTL clones were directed against two distinct antigens on the melanoma line. In view of the possibility that these antigens could be culture artefacts, we tested the stimulatory ability of tumor cells that had been freshly collected from metastatic relapses on the CTL clones. A considerable CTL proliferation was observed and it appeared to be specific. We conclude that the antigens recognized by the autologous CTL clones on the permanent melanoma cell line were expressed by the tumor cells in the patient.
一名黑色素瘤患者的外周血淋巴细胞在体外被源自自体肿瘤的永生细胞系刺激。获得了稳定的细胞毒性T淋巴细胞（CTL）克隆，这些克隆能够裂解黑色素瘤细胞系，但不能裂解自体爱泼斯坦-巴尔病毒转化的B淋巴细胞或K-562细胞。这些CTL克隆针对黑色素瘤细胞系上的两种不同抗原。鉴于这些抗原可能是培养假象的可能性，我们测试了从转移性复发中新鲜采集的肿瘤细胞对CTL克隆的刺激能力。观察到相当程度的CTL增殖，且似乎具有特异性。我们得出结论，自体CTL克隆在永久性黑色素瘤细胞系上识别的抗原在患者的肿瘤细胞中表达。
