80例“临床确诊”梅尼埃病患者体内的热休克蛋白70抗体
HSP70 antibodies in 80 patients with "clinically certain" Meniere's disease.
作者信息
Hornibrook Jeremy, George Peter, Spellerberg Myfanwy, Gourley John
机构信息
Department of Otolaryngology-Head and Neck Surgery and Audiology, Christchurch Hospital, and the Department of Communication Disorders, University of Canterbury, Christchurch, New Zealand.
出版信息
Ann Otol Rhinol Laryngol. 2011 Oct;120(10):651-5. doi: 10.1177/000348941112001004.
OBJECTIVES
We tested the claim that a significant proportion of patients with Meniere's disease have antibodies to heat shock protein 70 (HSP70) antigen, which may lead to defective endolymphatic sac function and vertigo attacks.
METHODS
Serum samples were taken from 80 subjects with a "certain" diagnosis of Meniere's disease (American Academy criteria plus electrocochleographic confirmation of endolymphatic hydrops with tone burst stimuli) and were tested for HSP70 antibodies with the OTOblot (hsp70) Western blot assay. The response was recorded as negative, positive, or equivocal. Samples from 80 sex- and age-matched blood donors were used as controls.
RESULTS
Of 80 patients with "clinically certain" Meniere's disease, 14 were positive for HSP70 antibodies or equivocal; of 80 controls, 10 were positive or equivocal. There was no significant difference (p=0.239). There was no correlation with bilateral disease, "activity" of Meniere's disease, or stage of Meniere's disease.
CONCLUSIONS
Patients with an unequivocal diagnosis of Meniere's disease do not have a significantly raised incidence of HSP70 antibodies.
目的
我们对以下观点进行了验证，即相当一部分梅尼埃病患者体内存在针对热休克蛋白70（HSP70）抗原的抗体，这可能导致内淋巴囊功能缺陷和眩晕发作。
方法
采集了80例经“明确”诊断为梅尼埃病的患者（符合美国耳鼻咽喉头颈外科学会标准且经电耳蜗图检查证实存在内淋巴积水伴短纯音刺激）的血清样本，采用OTOblot（hsp70）免疫印迹法检测HSP70抗体。结果记录为阴性、阳性或可疑。选取80例年龄和性别匹配的献血者样本作为对照。
结果
80例“临床确诊”的梅尼埃病患者中，14例HSP70抗体呈阳性或可疑；80例对照者中，10例呈阳性或可疑。两者无显著差异（p = 0.239）。与双侧患病、梅尼埃病的“活动度”或梅尼埃病分期均无相关性。
结论
明确诊断为梅尼埃病的患者中，HSP70抗体发生率并无显著升高。
Zotero 插件