Yang Fan, Zhou Xue-jun, Liu Nan-qiao, Zhang Yu, Wu Guo-liang, Cheng Yue, Huang Xi-wen, Cai Ya-fei, Wang Gen-lin
College of Life Sciences, Anhui Normal University, Wuhu 241000, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2011 Aug;27(3):333-7.
In order to study the effect of the polymorphism at the exon2 region of the (3-LG allele gene on milk composition and yield.
The single-strand conformation polymorphism method (PCR-SSCP) was used to analyze for polymorphism the exon2 region of the 3-LG gene (NCBI accession number: DQ489319) in Chinese Holstein.
Eight SSCP patterns were detected in the fragments: ab, abc, abd, abe, abcd, abce, abde and abcde, and the patterns frequencies as follows: 0.14, 0.10, 0.27, 0.23, 0.05, 0.04, 0.11 and 0.06 (P < 0.05); Six single nucleotide polymorphism (SNP) were detected in this study: sitel C>T, site2 T>C, site3 C>T, site4 C>C, site5 C> A, site6 A>T or C, and the polymorphism infonnation content (PIC) of these SNPs were in median or high polymorphism (PIC > 0.25).
These SNPs at the exon2 region of the beta-LG gene were remarkably and affected milk performance traits (milk yield, protein and fat contents) in Chinese Holstein.
