Yuan Weimin, Wu Xiaoqin, Ye Jianren, Tian Xiaojing
Nanjing Forestry University, Key Laboratory of Pest Prevention and Control of Jiangsu Province, Nanjing 210037, China.
Wei Sheng Wu Xue Bao. 2011 Aug;51(8):1071-7.
The pine wood nematode, Bursaphlenchus xylophilus, morphologically similar to B. mucronatus, is the pathogen of pine wilt disease. This study was focused on the endophytic bacteria present in these nematodes.
Detailed observations were made on sections of all parts of the two types of nematodes by transmission electron microscope. The nematodes were surface-sterilized by soaking in 1% mercuric chloride and antibiotic mixture, and then ground and cultured on nutrient agar plate. The physiological and biochemical characteristics combined with molecular characterization of bacteria were analyzed and identified.
Endophytic bacteria were found in intestines of the two nematodes by transmission electron microscope observations. On the basis of surface sterilization, total three bacteria strains were obtained from B. xylophilus and B. mucronatus. These bacteria belong to Stenotrophomonas and Ewingella.
It confirms the presence of endophytic bacteria in Bursaphelenchus xylophilus and B. mucronatus and these bacteria may play a physical and ecological roles in nematodes.
松材线虫（Bursaphlenchus xylophilus）与拟松材线虫（B. mucronatus）形态相似，是松材线虫病的病原体。本研究聚焦于这些线虫体内的内生细菌。
通过透射电子显微镜对两种线虫各部位切片进行详细观察。线虫经1%氯化汞和抗生素混合液浸泡进行表面消毒，然后研磨并接种于营养琼脂平板上培养。对细菌的生理生化特性结合分子特征进行分析鉴定。
通过透射电子显微镜观察在两种线虫的肠道中发现了内生细菌。经表面消毒后，从松材线虫和拟松材线虫中共获得三株细菌菌株。这些细菌属于嗜麦芽窄食单胞菌属（Stenotrophomonas）和尤因菌属（Ewingella）。
证实了松材线虫和拟松材线虫体内存在内生细菌，且这些细菌可能在线虫体内发挥生理和生态作用。
