Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu 210037, China.
Bureau of Parks and Woods, Huangshan Scenic Area Management Committee, Huangshan, Anhui, 245899, China.
Plant Dis. 2021 Oct;105(10):3055-3062. doi: 10.1094/PDIS-02-21-0396-RE. Epub 2021 Nov 7.
Pine wilt disease is the most devastating pine disease caused by . is morphologically similar to and geographically overlaps in its distribution. Although interspecific hybridization of the two nematodes has been performed in vitro, the dynamic regularity of hybrid formation and its risk in forests has not been well evaluated. In this study, a hybrid of and was identified in the laboratory and fields by molecular markers. The heterozygosity of ITS-5.8S loci for identification was unstable in the hybrid population, and the allele inherited from was lost over several generations. We also provided evidence that hybrids existed in some new epidemic areas, while old epidemic areas were usually dominated by . Hybrids could be generated when was invaded by , and the pathogenicity of the hybrids was similar to that of . These findings may improve the understanding of the natural hybridization between and and pathogenic variation in pine wilt disease, providing new insights for future studies on disease detection, transmission, and quarantine.
松材线虫病是由引起的最具破坏性的松树病害。在形态上与相似，在分布上也有重叠。尽管这两种线虫在体外已经进行了种间杂交，但它们在森林中的杂种形成的动态规律及其风险尚未得到很好的评估。在本研究中，通过分子标记在实验室和野外鉴定出了一种与的杂交种。ITS-5.8S 位点的杂合性对于鉴定是不稳定的，并且从遗传的等位基因在几代内丢失。我们还提供了证据表明，杂种存在于一些新的疫区，而旧疫区通常以为主。当入侵时，可能会产生杂种，并且杂种的致病性与相似。这些发现可能会提高对线虫和松材线虫病致病性变异之间自然杂交的理解，为未来关于疾病检测、传播和检疫的研究提供新的见解。
