Zhu Xiaofei, Zhang Xiaoxia, Niu Yongchun, Hu Yuansen, Yan Yanchun, Wang Haisheng
Institute of Bioengineering of Henan University of Technology, Zhengzhou 450001, China.
Wei Sheng Wu Xue Bao. 2011 Aug;51(8):1128-33.
An antagonistic bacterial strain YB-3 against Rhizoctonia solani was isolated from soils.
Antagonistic strains were isolated by a reporter strain method. YB-3 was identified based on morphology observation, physiological and biochemical characterizations, Biolog, G + C content and 16S rDNA sequence analysis. The antagonistic spectrum and the properties of the inhibitor produced by Bacillus amyloliquefaciens YB-3 against plant pathogenic fungi and bacteria were investigated by means of plate two-way cultivation and disc diffusion method.
The strain YB-3 against Rhizoctonia solani was identified as Bacillus amyloliquefaciens. The antagonistic results showed that it had distinctively inhibitive effects on 14 pathogenic fungi and 7 bacteria. In addition, it also had inhibitive effects on strains from genus Bacillus to which YB-3 belongs. Antagonistic properties of B. amyloliquefaciens YB-3 was thermostable, acid resistant, and protease sensitive.
Bacillus amyloliquefaciens YB-3 was isolated and characterized which had distinctively inhibitive effects on Rhizoctonia solani and had broad-spectrum, highly efficient to plant pathogens.
从土壤中分离出一株对茄丝核菌具有拮抗作用的细菌菌株YB - 3。
采用报告菌株法分离拮抗菌株。通过形态观察、生理生化特性、Biolog、G + C含量及16S rDNA序列分析对YB - 3进行鉴定。采用平板双向培养法和滤纸片扩散法研究解淀粉芽孢杆菌YB - 3对植物病原真菌和细菌产生的抑制剂的拮抗谱及性质。
对茄丝核菌具有拮抗作用的菌株YB - 3被鉴定为解淀粉芽孢杆菌。拮抗结果表明，它对14种病原真菌和7种细菌有明显的抑制作用。此外，它对YB - 3所属的芽孢杆菌属菌株也有抑制作用。解淀粉芽孢杆菌YB - 3的拮抗特性具有热稳定性、耐酸性和对蛋白酶敏感。
分离并鉴定了解淀粉芽孢杆菌YB - 3，其对茄丝核菌有明显抑制作用，对植物病原菌具有广谱、高效性。
