[一株抗立枯丝核菌的解淀粉芽孢杆菌YB-3的分离与鉴定]
[Isolation and identification of a Bacillus amyloliquefaciens YB-3 against Rhizoctonia solani].
作者信息
Zhu Xiaofei, Zhang Xiaoxia, Niu Yongchun, Hu Yuansen, Yan Yanchun, Wang Haisheng
机构信息
Institute of Bioengineering of Henan University of Technology, Zhengzhou 450001, China.
出版信息
Wei Sheng Wu Xue Bao. 2011 Aug;51(8):1128-33.
OBJECTIVE
An antagonistic bacterial strain YB-3 against Rhizoctonia solani was isolated from soils.
METHODS
Antagonistic strains were isolated by a reporter strain method. YB-3 was identified based on morphology observation, physiological and biochemical characterizations, Biolog, G + C content and 16S rDNA sequence analysis. The antagonistic spectrum and the properties of the inhibitor produced by Bacillus amyloliquefaciens YB-3 against plant pathogenic fungi and bacteria were investigated by means of plate two-way cultivation and disc diffusion method.
RESULTS
The strain YB-3 against Rhizoctonia solani was identified as Bacillus amyloliquefaciens. The antagonistic results showed that it had distinctively inhibitive effects on 14 pathogenic fungi and 7 bacteria. In addition, it also had inhibitive effects on strains from genus Bacillus to which YB-3 belongs. Antagonistic properties of B. amyloliquefaciens YB-3 was thermostable, acid resistant, and protease sensitive.
CONCLUSION
Bacillus amyloliquefaciens YB-3 was isolated and characterized which had distinctively inhibitive effects on Rhizoctonia solani and had broad-spectrum, highly efficient to plant pathogens.
目的
从土壤中分离出一株对茄丝核菌具有拮抗作用的细菌菌株YB - 3。
方法
采用报告菌株法分离拮抗菌株。通过形态观察、生理生化特性、Biolog、G + C含量及16S rDNA序列分析对YB - 3进行鉴定。采用平板双向培养法和滤纸片扩散法研究解淀粉芽孢杆菌YB - 3对植物病原真菌和细菌产生的抑制剂的拮抗谱及性质。
结果
对茄丝核菌具有拮抗作用的菌株YB - 3被鉴定为解淀粉芽孢杆菌。拮抗结果表明，它对14种病原真菌和7种细菌有明显的抑制作用。此外，它对YB - 3所属的芽孢杆菌属菌株也有抑制作用。解淀粉芽孢杆菌YB - 3的拮抗特性具有热稳定性、耐酸性和对蛋白酶敏感。
结论
分离并鉴定了解淀粉芽孢杆菌YB - 3，其对茄丝核菌有明显抑制作用，对植物病原菌具有广谱、高效性。
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