Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Nutr Cancer. 2012;64(1):80-8. doi: 10.1080/01635581.2012.630162. Epub 2011 Nov 18.
Scutellaria is a genus of Lamiaceae with known antiproliferative potentials. Scutellaria litwinowii Bornm. & Sint. ex Bornm. is one of the Iranian species of Scutellaria. Although there are widespread reports about the cytotoxic and antitumor effects of some species of this genus, research on the molecular mechanism responsible for the anticancer effects of S. litwinowii has not yet been conducted. In the current study, the apoptotic effects of S. litwinowii on 2 myeloid cell lines, apoptosis-proficient HL60 cells and apoptosis-resistant K562 cells, were analyzed. An increase in the activity of caspases-3, -8, and -9, poly (ADP ribose) polymerase (PARP) cleavage, detection of phosphatidylserine on the outer layer of cell membrane and sub-G1 peak in the flow cytometry histogram of treated cells, suggested the induction of apoptosis. S. litwinowii also increased the Bax/Bcl-2 ratio. It could be concluded that S. litwinowii induced apoptosis in both apoptosis-proficient and apoptosis-resistant leukemic cells and it might be considered as a novel candidate in the treatment of hematological malignancies.
黄芩是唇形科的一个属，具有已知的抗增殖潜力。Litwinowii 黄芩是伊朗黄芩属的一种。尽管有广泛的报道称该属的一些物种具有细胞毒性和抗肿瘤作用，但尚未对 Litwinowii 黄芩的抗癌作用的分子机制进行研究。在本研究中，分析了黄芩对 2 种髓系细胞系（凋亡敏感的 HL60 细胞和凋亡抵抗的 K562 细胞）的促凋亡作用。细胞经黄芩处理后，caspase-3、-8 和 -9 的活性增加，多聚（ADP 核糖）聚合酶（PARP）切割，细胞膜外层的磷脂酰丝氨酸检测以及流式细胞术细胞周期直方图中的 sub-G1 峰增加，提示细胞发生了凋亡。黄芩还增加了 Bax/Bcl-2 比值。由此可以得出结论，黄芩在凋亡敏感和凋亡抵抗的白血病细胞中诱导了细胞凋亡，黄芩可能被视为治疗血液系统恶性肿瘤的一种新型候选药物。
