克罗米芬诱导周期的人黄体中的促黄体生成素和人绒毛膜促性腺激素受体

Luteinizing hormone and human chorionic gonadotropin receptors in human corpora lutea from clomiphene citrate-induced cycles.

作者信息

Yeko T R, Khan-Dawood F S, Dawood M Y

机构信息

University of Illinois College of Medicine, Chicago.

出版信息

Fertil Steril. 1990 Oct;54(4):601-5.

PMID:2209880

Abstract

Midluteal phase corpora lutea (CL) obtained from women induced with 50 mg (n = 5), 100 mg (n = 5), and 150 mg (n = 5) of clomiphene citrate (CC) were measured for luteinizing hormone/human chorionic gonadotropin (LH/hCG) concentrations and cytosol progesterone (P) and 17 alpha-hydroxyprogesterone (17-OHP) and compared with midluteal phase CL from eight normal women (controls). More CL (26) that were significantly heavier (2.0 +/- 0.3 g, [mean +/- SEM]) were obtained with CC than in controls (10). Clomiphene citrate treatment increased LH/hCG receptor concentrations and the dissociation constant significantly from 69 +/- 12 fmol/mg protein and 1.1 +/- 0.2 x 10(-10) M, respectively, in controls to 112 +/- 6 fmol/mg protein and 2.1 +/- 0.1 X 10(-10) M. Cytosol P and 17-OHP levels were not significantly increased. Cumulatively these cellular effects may be responsible for increasing serum P and responsiveness to hCG and for correcting luteal dysfunction.

摘要

从接受50毫克（n = 5）、100毫克（n = 5）和150毫克（n = 5）枸橼酸氯米芬（CC）诱导的女性中获取黄体中期的黄体（CL），测量其促黄体生成素/人绒毛膜促性腺激素（LH/hCG）浓度以及胞质孕酮（P）和17α-羟孕酮（17-OHP），并与8名正常女性（对照组）的黄体中期CL进行比较。与对照组（10个）相比，CC诱导获得的CL数量更多（26个），且明显更重（2.0±0.3克，[平均值±标准误]）。枸橼酸氯米芬治疗使LH/hCG受体浓度和离解常数显著增加，分别从对照组的69±12飞摩尔/毫克蛋白和1.1±0.2×10⁻¹⁰摩尔增加到112±6飞摩尔/毫克蛋白和2.1±0.1×10⁻¹⁰摩尔。胞质P和17-OHP水平未显著升高。这些细胞效应可能共同导致血清P升高、对hCG的反应性增强，并纠正黄体功能障碍。