Wu Fan, Li Guo-Lin, Song Xiao-Hua, Su Hai, Cao Xiao-Chun, Liu Yan-Ling, Ye Chun-Feng
Department of Pediatrics, Second Affiliated Hospital of Nanchang University, Nanchang, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2011 Nov;13(11):883-5.
To study the relationship between insertion/deletion (I/D) polymorphism of 287 bp in the 16th intron of angiotensin converting enzyme (ACE) and essential hypertension in children.
I/D polymorphism of 287 bp in the 16th intron of ACE was detected using PCR in 105 children with essential hypertension and 105 normal children as control group.
There was an I/D polymorphism in the 16th intron of ACE in the hypertension and the control groups: type II, type ID and type DD. The genotype frequencies of type DD, type ID and type II in the hypertension group were 30.5%, 47.6% and 21.9%, respectively. The genotype frequencies of type DD, type ID and type II in the control group were 14.3%, 46.7% and 39.1%, respectively. There were significant differences in the genotype frequencies of types DD and II between the two groups (P<0.01). The allele frequency of type D (54.3% vs 37.6%) was significantly higher in the hypertension group; in contrast, the allele frequency of type I (45.7% vs 62.4%) was significantly lower than in the control group (P<0.01).
Polymorphism of type II, type ID and type DD exits in ACE. The deletion of 287 bp in the 16th intron of ACE might be associated with the occurrence of essential hypertension in children.
研究血管紧张素转换酶（ACE）第16内含子287 bp插入/缺失（I/D）多态性与儿童原发性高血压的关系。
采用聚合酶链反应（PCR）检测105例儿童原发性高血压患者及105例正常儿童（对照组）ACE第16内含子287 bp的I/D多态性。
高血压组和对照组ACE第16内含子均存在I/D多态性，即II型、ID型和DD型。高血压组DD型、ID型和II型的基因型频率分别为30.5%、47.6%和21.9%。对照组DD型、ID型和II型的基因型频率分别为14.3%、46.7%和39.1%。两组间DD型和II型的基因型频率存在显著差异（P<0.01）。高血压组D等位基因频率（54.3%对37.6%）显著高于对照组；相反，I等位基因频率（45.7%对62.4%）显著低于对照组（P<0.01）。
ACE存在II型、ID型和DD型多态性。ACE第16内含子287 bp的缺失可能与儿童原发性高血压的发生有关。
