Department of Materials, School of Engineering & Materials Science, Queen Mary University of London, London E1 4NS, UK.
J Mech Behav Biomed Mater. 2012 Jan;5(1):149-55. doi: 10.1016/j.jmbbm.2011.08.018. Epub 2011 Sep 13.
The mechanical properties of rat bone at micron length scales have been evaluated as a function of environmental conditions using an in situ atomic force microscope (AFM) setup while observing using scanning electron microscopy (SEM). Focused ion beam fabricated rat bone cantilever samples were tested in both low and high vacuum conditions in the SEM as well as wet in air using the AFM to measure their elastic modulus. The elastic modulus of rat bone at micron length scales is shown to be independent of the environmental testing conditions and indicates water is bound to bone material even under relatively high vacuum conditions. Our work therefore shows how in situ mechanical testing of bone while observing using high resolution SEM can provide results similar to testing wet in air.
已经使用原位原子力显微镜（AFM）设置评估了大鼠骨在微米长度尺度上的力学性能，同时使用扫描电子显微镜（SEM）进行观察，其功能取决于环境条件。使用聚焦离子束制造的大鼠骨悬臂梁样品在 SEM 中的低真空和高真空条件以及在空气中的湿条件下进行测试，使用 AFM 测量它们的弹性模量。结果表明，大鼠骨在微米长度尺度上的弹性模量与环境测试条件无关，这表明即使在相对高的真空条件下，水也与骨材料结合。因此，我们的工作表明，在使用高分辨率 SEM 进行原位力学测试的同时观察可以提供类似于在空气中湿测的结果。
