State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, People's Republic of China.
Acta Biomater. 2012 Feb;8(2):763-71. doi: 10.1016/j.actbio.2011.11.002. Epub 2011 Nov 9.
Electrospun nanofibers mimic the native extracellular matrix of bone and have generated considerable interest in bone tissue regeneration. The aim of this study was to fabricate novel poly(l-lactide-co-caprolactone) (PLLACL), PLLACL/collagen nanofibers blended with bone morphogenetic protein 2 (BMP2) and dexamethasone (DEX) for controlled release during bone tissue engineering (BTE). The morphology, surface hydrophilicity, and mechanical properties of the PLLACL/collagen nanofibrous mats were analyzed by scanning electron microscopy and water contact angle and mechanical stability determination. The performance of the scaffolds was investigated in terms of the viability and morphology of human mesenchymal stromal cells (hMSC) on the nanofibrous mats. BMP2 and DEX were successfully incorporated into PLLACL/collagen nanofibers by means of blending or coaxial electrospinning and the PLLACL/collagen blended fibers proved useful for hMSC culture. Release of the two growth factors from PLLACL/collagen nanofibrous mats in vitro was investigated by UV spectrophotometry. The release profiles for core-shell nanofibers showed more controlled release of the growth factors compared with the blended electrospun fibers. The experimental results show that controlled release of BMP2 and DEX can induce hMSC to differentiate into osteogenic cells for bone tissue engineering. The results imply that PLLACL/collagen nanofibers encapsulating two drugs and/or proteins have great potential in bone tissue engineering.
静电纺丝纳米纤维模拟了骨的天然细胞外基质，在骨组织再生方面引起了极大的兴趣。本研究的目的是制备新型聚（L-丙交酯-共-己内酯）（PLLACL）、PLLACL/胶原纳米纤维，与骨形态发生蛋白 2（BMP2）和地塞米松（DEX）混合，用于骨组织工程（BTE）期间的控制释放。通过扫描电子显微镜和水接触角以及机械稳定性测定来分析 PLLACL/胶原纳米纤维垫的形态、表面亲水性和机械性能。通过考察纳米纤维垫上人间充质基质细胞（hMSC）的活力和形态，研究了支架的性能。通过共混或同轴静电纺丝成功将 BMP2 和 DEX 掺入 PLLACL/胶原纳米纤维中，并且 PLLACL/胶原共混纤维对 hMSC 培养很有用。通过紫外分光光度法研究了 PLLACL/胶原纳米纤维垫中两种生长因子的体外释放情况。与共混静电纺丝纤维相比，核壳纳米纤维的释放曲线显示出对生长因子更可控的释放。实验结果表明，BMP2 和 DEX 的控制释放可以诱导 hMSC 分化为成骨细胞，用于骨组织工程。结果表明，包封两种药物和/或蛋白质的 PLLACL/胶原纳米纤维在骨组织工程中有很大的潜力。
