Viel A, Armand M J, Callen J C, Gomez De Gracia A, Denis H, le Maire M
Centre de Génétique Moléculaire, Laboratoire propre du CNRS, Université P. et M. Curie (Paris VI), Gif-sur-Yvette, France.
Dev Biol. 1990 Oct;141(2):270-8. doi: 10.1016/0012-1606(90)90383-t.
In Xenopus laevis oocytes two distinct systems catalyze the mRNA-dependent binding of aminoacyl tRNA to the A site of ribosomes. These systems are elongation factor 1 alpha (EF-1 alpha) and the 42S nucleoprotein particle. This particle is also implicated in the long-term storage of 5S RNA and aminoacyl tRNA during early oogenesis. We report here that the ribosomes and the storage particles are distributed uniformly in the cytoplasm of previtellogenic (stage I) oocytes. In contrast, EF-1 alpha is concentrated in a small region of the cytoplasm, known as the mitochondrial mass or Balbiani body. When the Balbiani body disperses in early vitellogenic oocytes (stage II), EF-1 alpha becomes evenly distributed in the cytoplasm. The main phase of EF-1 alpha accumulation follows the disappearance of the 42S particles (stage II), but coincides with the main phase of ribosome accumulation (stages III and IV).
在非洲爪蟾卵母细胞中，有两个不同的系统催化氨酰tRNA依赖mRNA与核糖体A位点的结合。这些系统是延伸因子1α（EF-1α）和42S核蛋白颗粒。该颗粒也与早期卵子发生过程中5S RNA和氨酰tRNA的长期储存有关。我们在此报告，核糖体和储存颗粒在前卵黄生成期（I期）卵母细胞的细胞质中均匀分布。相比之下，EF-1α集中在细胞质的一个小区域，即线粒体团或巴尔比亚尼体。当巴尔比亚尼体在早期卵黄生成期卵母细胞（II期）中分散时，EF-1α在细胞质中均匀分布。EF-1α积累的主要阶段在42S颗粒消失后（II期），但与核糖体积累的主要阶段（III期和IV期）一致。
