两种形式的延伸因子1α（EF-1αO和42Sp50）存在于非洲爪蟾卵母细胞中，但在其体细胞中不存在。
Two forms of elongation factor 1 alpha (EF-1 alpha O and 42Sp50), present in oocytes, but absent in somatic cells of Xenopus laevis.
作者信息
Deschamps S, Morales J, Mazabraud A, le Maire M, Denis H, Brown D D
机构信息
Centre de Génétique Moléculaire, Université Pierre et Marie Curie, Gif-sur-Yvette, France.
出版信息
J Cell Biol. 1991 Sep;114(6):1109-11. doi: 10.1083/jcb.114.6.1109.
Abstract
We have purified and partially sequenced the EF-1 alpha protein from Xenopus laevis oocytes (EF-1 alpha O). We show that the two cDNA clones isolated by Coppared et al. (Coppard, N. J., K. Poulsen, H. O. Madsen, J. Frydenberg, and B. F. C. Clark. 1991. J. Cell Biol. 112:237-243) do not encode 42Sp50, as claimed by these authors, but two very similar forms of EF-1 alpha O (EF-1 alpha O and EF-1 alpha O1). 42Sp50 is the major protein component of a 42S nucleoprotein particle that is very abundant in previtellogenic oocytes of X. laevis, 42Sp50 differs from EF-1 alpha O not only by its amino acid sequence, but also by several properties already reported. In particular, 42Sp50 has a low EF-1 alpha activity. It is distributed uniformly in the cytoplasm of previtellogenic oocytes, in contrast to EF-1 alpha O which is concentrated in a small region of the cytoplasm, known as the mitochondrial mass or Balbiani body.
摘要
我们已经从非洲爪蟾卵母细胞中纯化了延伸因子1α蛋白（EF-1αO）并对其进行了部分测序。我们发现，Coppared等人（Coppard, N. J., K. Poulsen, H. O. Madsen, J. Frydenberg, and B. F. C. Clark. 1991. J. Cell Biol. 112:237-243）分离得到的两个cDNA克隆并不像这些作者所声称的那样编码42Sp50，而是编码两种非常相似的EF-1αO形式（EF-1αO和EF-1αO1）。42Sp50是一种42S核蛋白颗粒的主要蛋白质成分，在非洲爪蟾的卵黄生成前卵母细胞中非常丰富，42Sp50不仅在氨基酸序列上与EF-1αO不同，而且在一些已报道的特性上也有所不同。特别是，42Sp50具有较低的EF-1α活性。它均匀地分布在卵黄生成前卵母细胞的细胞质中，与之形成对比的是，EF-1αO集中在细胞质的一个小区域，即线粒体团或巴尔比亚尼小体。
相似文献
1
Two forms of elongation factor 1 alpha (EF-1 alpha O and 42Sp50), present in oocytes, but absent in somatic cells of Xenopus laevis.两种形式的延伸因子1α（EF-1αO和42Sp50）存在于非洲爪蟾卵母细胞中，但在其体细胞中不存在。
J Cell Biol. 1991 Sep;114(6):1109-11. doi: 10.1083/jcb.114.6.1109.
2
Three genes under different developmental control encode elongation factor 1-alpha in Xenopus laevis.非洲爪蟾中，三个受不同发育调控的基因编码延伸因子1-α。
Nucleic Acids Res. 1990 Jun 25;18(12):3489-93. doi: 10.1093/nar/18.12.3489.
3
Elongation factor 1 alpha (EF-1 alpha) is concentrated in the Balbiani body and accumulates coordinately with the ribosomes during oogenesis of Xenopus laevis.延伸因子1α（EF-1α）集中在巴尔比亚尼小体中，并在非洲爪蟾卵子发生过程中与核糖体协同积累。
Dev Biol. 1990 Oct;141(2):270-8. doi: 10.1016/0012-1606(90)90383-t.
4
Structural and functional properties of thesaurin a (42Sp50), the major protein of the 42 S particles present in Xenopus laevis previtellogenic oocytes.非洲爪蟾卵黄发生前期卵母细胞中42S颗粒的主要蛋白质——鞘蛋白a（42Sp50）的结构与功能特性。 <br> （注：原文中thesaurin a推测可能是saurin a，中文译文根据推测进行了相应翻译。若thesaurin a有准确特定含义，可根据实际情况调整。）
J Biol Chem. 1991 Jun 5;266(16):10392-9.
5
42Sp48 in previtellogenic Xenopus oocytes is structurally homologous to EF-1 alpha and may be a stage-specific elongation factor.非洲爪蟾卵黄生成前的卵母细胞中的42Sp48在结构上与延伸因子1α同源，可能是一种阶段特异性延伸因子。
J Cell Biol. 1991 Jan;112(2):237-43. doi: 10.1083/jcb.112.2.237.
6
Isolation and characterization of the gene encoding EF-1 alpha O, an elongation factor 1-alpha expressed during early development of Xenopus laevis.非洲爪蟾早期发育过程中表达的延伸因子1-α（EF-1αO）编码基因的分离与鉴定。
Gene. 1991 Dec 30;109(2):185-92. doi: 10.1016/0378-1119(91)90608-e.
7
Visualizing protein interactions involved in the formation of the 42S RNP storage particle of Xenopus oocytes.可视化爪蟾卵母细胞中 42S RNP 储存颗粒形成过程中涉及的蛋白质相互作用。
Biol Cell. 2010 May 26;102(8):469-78. doi: 10.1042/BC20100034.
8
42S p48--the most abundant protein in previtellogenic Xenopus oocytes--resembles elongation factor 1 alpha structurally and functionally.42S p48（非洲爪蟾卵黄生成前卵母细胞中最丰富的蛋白质）在结构和功能上类似于延伸因子1α。
EMBO J. 1987 Aug;6(8):2409-13. doi: 10.1002/j.1460-2075.1987.tb02519.x.
9
A purified complex from Xenopus oocytes contains a p47 protein, an in vivo substrate of MPF, and a p30 protein respectively homologous to elongation factors EF-1 gamma and EF-1 beta.
FEBS Lett. 1989 Sep 11;255(1):101-4. doi: 10.1016/0014-5793(89)81069-5.
10
Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes.在非洲爪蟾卵母细胞中，42Sp50 与植物 RNA 定位机制的相互作用。
FEBS J. 2010 Nov;277(22):4722-31. doi: 10.1111/j.1742-4658.2010.07878.x. Epub 2010 Oct 6.
引用本文的文献
1
Amphibian Egg Jelly as a Biocompatible Material: Physicochemical Characterization and Selective Cytotoxicity Against Melanoma Cells.两栖动物卵胶作为一种生物相容性材料：物理化学特性及对黑色素瘤细胞的选择性细胞毒性
Polymers (Basel). 2025 Jul 27;17(15):2046. doi: 10.3390/polym17152046.
2
Expression and regulation of in spotted scat ().点带石斑鱼（）中 的表达与调控 。
你提供的原文中“Expression and regulation of in spotted scat ()”似乎有信息缺失，括号里应该有具体的研究对象等内容。
Front Genet. 2022 Aug 11;13:964150. doi: 10.3389/fgene.2022.964150. eCollection 2022.
3
First account of a transient intersex in spotted scat, Scatophagus argus: a marine gonochoristic fish.首例斑点鲷（Scatophagus argus）中间性个体的报道：一种海洋雌雄同体鱼类。
Fish Physiol Biochem. 2022 Aug;48(4):1011-1023. doi: 10.1007/s10695-022-01097-x. Epub 2022 Jul 8.
4
Genome-Wide Identification, Evolution and Expression of the Complete Set of Cytoplasmic Ribosomal Protein Genes in Nile Tilapia.尼罗罗非鱼细胞质核糖体蛋白基因家族的全基因组鉴定、进化和表达分析。
Int J Mol Sci. 2020 Feb 12;21(4):1230. doi: 10.3390/ijms21041230.
5
Heterozygous mutation of eEF1A1b resulted in spermatogenesis arrest and infertility in male tilapia, Oreochromis niloticus.异源突变的 eEF1A1b 导致雄性罗非鱼（奥利亚罗非鱼）的精子发生停滞和不育。
Sci Rep. 2017 Mar 7;7:43733. doi: 10.1038/srep43733.
6
Distribution of elongation factor-1alpha in larval tissues of the fall armyworm, Spodoptera frugiperda.伸长因子-1α在草地贪夜蛾幼虫组织中的分布
J Insect Sci. 2006;6:1-9. doi: 10.1673/2006_06_33.1.
7
Oscheius tipulae as an example of eEF1A gene diversity in nematodes.以奥氏线虫为例探讨线虫中延伸因子1A（eEF1A）基因的多样性。
J Mol Evol. 2008 Sep;67(3):278-90. doi: 10.1007/s00239-008-9147-8. Epub 2008 Aug 12.
8
Scythe regulates apoptosis through modulating ubiquitin-mediated proteolysis of the Xenopus elongation factor XEF1AO.Scythe通过调节泛素介导的非洲爪蟾延伸因子XEF1AO的蛋白水解作用来调控细胞凋亡。
Biochem J. 2007 Aug 1;405(3):495-501. doi: 10.1042/BJ20061886.
本文引用的文献
1
Biochemical Research on oogenesis. Composition of the 42-S storage particles of Xenopus laevix oocytes.卵子发生的生化研究。非洲爪蟾卵母细胞42-S储存颗粒的组成。
Eur J Biochem. 1980 Aug;109(2):359-68. doi: 10.1111/j.1432-1033.1980.tb04802.x.
2
Nuclear exclusion of transcription factor IIIA and the 42s particle transfer RNA-binding protein in Xenopus oocytes: a possible mechanism for gene control?非洲爪蟾卵母细胞中转录因子IIIA和42S颗粒转运RNA结合蛋白的核排除：一种可能的基因调控机制？
J Cell Biol. 1983 Oct;97(4):1261-5. doi: 10.1083/jcb.97.4.1261.
3
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
4
Thesaurin a, the major protein of Xenopus laevis previtellogenic oocytes, present in the 42 S particles, is homologous to elongation factor EF-1 alpha.非洲爪蟾前卵黄生成卵母细胞的主要蛋白质Thesaurin a存在于42 S颗粒中，与延伸因子EF-1α同源。
FEBS Lett. 1987 Nov 2;223(2):232-6. doi: 10.1016/0014-5793(87)80295-8.
5
Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.从电转印到聚偏二氟乙烯膜上的皮摩尔量蛋白质中获得的序列。
J Biol Chem. 1987 Jul 25;262(21):10035-8.
6
Peptide elongation factor 1 from yeasts: purification and biochemical characterization of peptide elongation factors 1 alpha and 1 beta (gamma) from Saccharomyces carlsbergensis and Schizosaccharomyces pombe.酵母中的肽链延伸因子1：来自卡尔斯伯酵母和粟酒裂殖酵母的肽链延伸因子1α和1β（γ）的纯化及生化特性分析
J Biochem. 1988 Mar;103(3):508-21. doi: 10.1093/oxfordjournals.jbchem.a122301.
7
The mRNA encoding elongation factor 1-alpha (EF-1 alpha) is a major transcript at the midblastula transition in Xenopus.编码延伸因子1-α（EF-1α）的信使核糖核酸是非洲爪蟾囊胚中期转换时的一种主要转录物。
Dev Biol. 1989 May;133(1):93-100. doi: 10.1016/0012-1606(89)90300-x.
8
42S p48--the most abundant protein in previtellogenic Xenopus oocytes--resembles elongation factor 1 alpha structurally and functionally.42S p48（非洲爪蟾卵黄生成前卵母细胞中最丰富的蛋白质）在结构和功能上类似于延伸因子1α。
EMBO J. 1987 Aug;6(8):2409-13. doi: 10.1002/j.1460-2075.1987.tb02519.x.
9
Three genes under different developmental control encode elongation factor 1-alpha in Xenopus laevis.非洲爪蟾中，三个受不同发育调控的基因编码延伸因子1-α。
Nucleic Acids Res. 1990 Jun 25;18(12):3489-93. doi: 10.1093/nar/18.12.3489.
10
Two different mRNAs coding for identical elongation factor 1 alpha (EF-1 alpha) polypeptides in Xenopus laevis embryos.非洲爪蟾胚胎中编码相同延伸因子1α（EF-1α）多肽的两种不同信使核糖核酸。
Differentiation. 1990 Aug;44(2):103-10. doi: 10.1111/j.1432-0436.1990.tb00543.x.
Zotero 插件