Unidad Médico-Quirúrgica de Enfermedades Respiratorias, Hospital Universitario Virgen del Rocío, Sevilla, Spain.
Respir Physiol Neurobiol. 2012 Jan 15;180(1):119-25. doi: 10.1016/j.resp.2011.11.001. Epub 2011 Nov 9.
We sought to investigate whether the serum concentrations of several inflammatory biomarkers are related to the cyclooxygenase-2 (COX2) -765G>C polymorphism in chronic obstructive pulmonary disease (COPD) and a control group of non-COPD smokers. Serum inflammatory markers (CRP, SAA, CXCL8, and sICAM-1) were measured by ELISA in 144 patients with COPD and in 55 control subjects. Genomic DNA was extracted from peripheral blood leukocytes, and the COX2 -765G>C (rs20417) polymorphism was genotyped. After adjustment for age and active smoking, CRP and SAA concentrations were associated with the COX2 polymorphism in controls (p=0.041 and 0.014, respectively) but not in COPD patients. The CXCL8 and sICAM-1 concentrations were not associated with the COX2 polymorphism for either cases or controls. The results of the present study indicate that there is a relationship between the COX2 -765G>C polymorphism and the concentrations of CRP and SAA in non-COPD smokers and that this relationship does not exist in COPD patients.
我们试图研究几种炎症生物标志物的血清浓度是否与慢性阻塞性肺疾病（COPD）患者和非 COPD 吸烟对照组中的环氧化酶-2（COX2）-765G>C 多态性有关。通过酶联免疫吸附试验（ELISA）测定了 144 例 COPD 患者和 55 例对照组患者的血清炎症标志物（CRP、SAA、CXCL8 和 sICAM-1）。从外周血白细胞中提取基因组 DNA，并对 COX2-765G>C（rs20417）多态性进行基因分型。在调整年龄和主动吸烟后，CRP 和 SAA 浓度与对照组的 COX2 多态性相关（分别为 p=0.041 和 0.014），但与 COPD 患者无关。对于病例组和对照组，CXCL8 和 sICAM-1 浓度与 COX2 多态性均无关。本研究结果表明，COX2-765G>C 多态性与非 COPD 吸烟者的 CRP 和 SAA 浓度之间存在关联，而在 COPD 患者中则不存在这种关联。
