胚胎心脏间充质增殖的调控：转化生长因子-β1和间质基质的作用

Regulation of proliferation of embryonic heart mesenchyme: role of transforming growth factor-beta 1 and the interstitial matrix.

作者信息

Choy M, Armstrong M T, Armstrong P B

机构信息

Department of Pediatrics, University of California Davis Medical Center, Sacramento 95817.

出版信息

Dev Biol. 1990 Oct;141(2):421-5. doi: 10.1016/0012-1606(90)90396-z.

DOI:10.1016/0012-1606(90)90396-z

PMID:2210044

Abstract

Proliferation of atrioventricular cushion mesenchyme of the embryonic avian heart maintained in three-dimensional aggregate culture is stimulated by interaction with the interstitial matrix. Chicken serum or transforming growth factor-beta 1, which stimulates proliferation, induces matrix deposition in regions of the aggregate showing high labeling indices with tritiated thymidine. Dispersed heart mesenchyme interstitial matrix introduced into serum-free culture is incorporated into the aggregate and stimulates cellular proliferation similar to serum or transforming growth factor-beta 1. Proliferation is reversibly inhibited by the peptide Gly-Arg-Gly-Asp-Ser-Pro. It is suggested that transforming growth factor-beta 1 stimulates the production of interstitial matrix and that a sufficient stimulus for proliferation in this system is the presence of the matrix, which acts as the adhesive support for cellular anchorage.

摘要

在三维聚集体培养中，胚胎鸡心脏房室垫间充质与细胞外基质相互作用可刺激其增殖。刺激增殖的鸡血清或转化生长因子-β1会诱导聚集体中标记指数高的区域出现基质沉积，这些区域用氚标记胸腺嘧啶核苷进行检测。引入无血清培养的分散心脏间充质细胞外基质会整合到聚集体中，并刺激细胞增殖，效果与血清或转化生长因子-β1相似。增殖可被肽Gly-Arg-Gly-Asp-Ser-Pro可逆性抑制。研究表明，转化生长因子-β1刺激细胞外基质的产生，且该系统中增殖的充分刺激因素是基质的存在，基质作为细胞锚定的黏附支持物发挥作用。