Nakashima K, Hattori Y, Yamazaki K, Takechi M, Andoh Y, Miyaji T
Department of Laboratory Medicine, St. Luke's College of Nursing, Tokyo, Japan.
Diabetes. 1990 Jan;39(1):17-21. doi: 10.2337/diacare.39.1.17.
Our aim was to find a simple method of removing labile glycosylated hemoglobin (HbA1c) from blood samples before it is measured by cation-exchange chromatography. Labile HbA1c is formed by the binding of glucose to the NH2-terminal valine of the beta-chain of HbA. We sought a more competitive binder for the same site to dissociate labile HbA1c to glucose and HbA. Inorganic phosphates were found to have a strong allosteric effect and a great ability to eliminate labile HbA1c. We developed our method with 4 mM tetrapolyphosphate in the hemolyzing solution to eliminate labile HbA1c during the automatic processing (at pH 6 and heated for 2 min at 45 degrees C) of blood samples for HbA1c estimation. This may be useful when estimating HbA1c by the manual method.
我们的目标是找到一种简单的方法，在通过阳离子交换色谱法测量血样之前，去除其中不稳定的糖化血红蛋白（HbA1c）。不稳定的HbA1c是由葡萄糖与HbAβ链的NH2末端缬氨酸结合形成的。我们寻找一种对同一位点具有更强竞争力的结合剂，以使不稳定的HbA1c解离为葡萄糖和HbA。发现无机磷酸盐具有很强的变构效应和消除不稳定HbA1c的强大能力。我们开发了一种方法，在溶血溶液中加入4 mM的四聚磷酸盐，以便在自动处理（pH值为6，在45摄氏度加热2分钟）血样以进行HbA1c估计的过程中消除不稳定的HbA1c。在通过手动方法估计HbA1c时，这可能会很有用。
