Adkins-Marshall B A, Myers S R, Hendrick G K, Williams P E, Triebwasser K, Floyd B, Cherrington A D
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0615.
Diabetes. 1990 Jan;39(1):87-95. doi: 10.2337/diacare.39.1.87.
In the presence of fixed basal levels of insulin, the route of intravenous glucose delivery (protal vs. peripheral) determines whether net hepatic glucose uptake (NHGU) occurs. Our aims were to determine if the route of intravenous glucose delivery also plays a role in regulating NHGU in the presence of hyperinsulinemia and to determine if length of fast (18 vs. 36 h) influences regulation of NHGU. Five conscious dogs fasted 18 h were given somatostatin and replacement insulin (245 +/- 34 microU.kg-1.min-1) and glucagon (0.65 ng.kg-1.min-1) infusions intraportally. After a 40-min control period, the insulin infusion rate was increased fourfold, and glucose was infused for 3 h. Glucose was given either through a peripheral vein or the portal vein for 90 min to double the glucose load reaching the liver. The order of infusions was randomized. NHGU was measured with the arterial - venous difference technique. Insulin and glucagon levels were 12 +/- 2, 35 +/- 6, and 36 +/- 5 microU/ml and 55 +/- 12, 61 +/- 13, and 59 +/- 7 pg/ml during the control, peripheral, and portal infusions, respectively. The glucose infusion rate, the load of glucose reaching the liver, and the arterial-portal plasma glucose gradient were 0, 9.58 +/- 2.28, and 10.44 +/- 2.94 mg.kg-1.min-1; 29.4 +/- 3.6, 56.8 +/- 3.4, and 56.8 +/- 2.8 mg.kg-1.min-1; and 2 +/- 1, 5 +/- 1, and -51 +/- 15 mg/dl during the same periods.(ABSTRACT TRUNCATED AT 250 WORDS)
在胰岛素基础水平固定的情况下，静脉输注葡萄糖的途径（门静脉与外周静脉）决定了肝脏是否发生净葡萄糖摄取（NHGU）。我们的目的是确定在高胰岛素血症情况下静脉输注葡萄糖的途径是否也在调节NHGU中起作用，以及禁食时间（18小时与36小时）是否影响NHGU的调节。对5只禁食18小时的清醒犬经门静脉给予生长抑素、补充胰岛素（245±34微单位·千克⁻¹·分钟⁻¹）和胰高血糖素（0.65纳克·千克⁻¹·分钟⁻¹）输注。在40分钟的对照期后，将胰岛素输注速率提高四倍，并输注葡萄糖3小时。葡萄糖通过外周静脉或门静脉输注90分钟，以使到达肝脏的葡萄糖负荷加倍。输注顺序是随机的。用动静脉差技术测量NHGU。在对照、外周和门静脉输注期间，胰岛素和胰高血糖素水平分别为12±2、35±6和36±5微单位/毫升以及55±12、61±13和59±7皮克/毫升。在相同期间，葡萄糖输注速率、到达肝脏的葡萄糖负荷以及动脉-门静脉血浆葡萄糖梯度分别为0、9.58±2.28和10.44±2.94毫克·千克⁻¹·分钟⁻¹；29.4±3.6、56.8±3.4和56.8±2.8毫克·千克⁻¹·分钟⁻¹；以及2±1、5±1和-51±15毫克/分升。（摘要截断于250字）
